FENG Jinª²Hua1, LI Yuª²Guang1, SHI Gangª²Gang2, HOU Yuª²Qing3, CAO Shiª²Ping3

    1Department of Cardiology, First Affiliate Hospital, 2Department of Pharmacology, Medical College, Shantou University, Shantou 515041, China, 3Department of Cardiology, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, China

    ¡¾Abstract¡¿ AIM: To explore the possible effects of granulocyte colonyª²stimulating factor (Gª²CSF) on the homing of CD34+ bone marrow stem cell (BMSC) to myocardium. METHODS: Seventeen adult mongrel dogs were randomly assigned to 4 groups. Group 1 (Gª²CSF and MI, n=5) were treated with 10 ¦Ìg/(kg¡¤d)¡Á5 d of Gª²CSF injection sc and then myocardial infract operation; group 2 (Gª²CSF and sham operation, n=4), treated with 10 ¦Ìg/(kg¡¤d)¡Á5 d of Gª²CSF injection sc and sham operation; group 3 (nonª²Gª²CSF and MI, n=4) were injected with 10 ¦Ìg/(kg¡¤d)¡Á5 d sc normal saline and underwent myocardial infarct operation; group 4 (nonª²Gª²CSF and sham operation, n=4) were injected with 10 ¦Ìg/(kg¡¤d)¡Á5 d sc normal saline and get sham operation. Thoracotomy was performed on the left thorax of each animal and left anterior descending artery was exposed and separated about 1 cm. In group 1 and 3 the separated arteries were occluded for 3 h and then reª²opened, while the other 2 groups were not treated in this way. Every dogs received catheterizing in right femoral artery into the root of aorta arch and a topª²modified pigtail catheter cannulating through left jugular superficial vein into coronary venous sinus. 5 mL blood from each animal aorta and coronary sinus were harvested for two times, in which erythrocytes were lysed. Mononuclear cells were separated with lymphocyte separating solution and CD34+ BMSC were clarified and counted by flow cytometry. Data were calculated and managed with SPSS 10.0 statistical package. RESULTS: The amount of the CD34+ stem cells homing to myocardium in each group were (183.0¡À9.2)¡Á104/L, (71.1¡À9.2)¡Á104/L, (82.3¡À9.5)¡Á103/L and (78.5¡À9.8)¡Á103/L, respectively. We found after comparing the data of each group that the difference among every group was very significant (P=0.000, respectively) except between group 3 and 4 (P=0.912). CONCLUSION: Gª²CSF could not only vastly mobilize the BMSC into periphery blood, but also could increase the CD34+ stem cells homing to myocardium independent of myocardial infarct. ......