[摘要] 目的 探讨低能量激光照射联合龙葵多糖对荷肝癌小鼠肿瘤增殖的影响。方法 建立荷肝癌小鼠皮下种植瘤模型，将荷瘤小鼠随机分为5组，连续治疗10天后将小鼠处死。原位杂交法检测瘤组织血管内皮生长因子(VEGF)mRNA表达；免疫组化法检测瘤组织中细胞增殖核抗原Ki 67的表达。结果 低能量激光照射联合龙葵多糖可以抑制肿瘤细胞增殖及血管生成，显著下调VEGF mRNA 、Ki 67的表达。结论 低能量激光照射联合龙葵多糖能抑制肿瘤细胞的增殖及血管生成、抑制荷肝癌小鼠肿瘤的生长。

    [关键词] 低能量激光照射；龙葵多糖； 增殖

    Effects of laser irradiation solanum nigtum polysaccharide on tumor growth in cancer loaded mice

    NIE Qiao-zhen, HAN Yi-lin, SU Xiu-lan.The Affiliated Hospital of Inner Mongolia Medical College, Inner Mongolia,Hohhot 010050, China

    [Abstract] Objective To study effects of low energy laser irradiation combined solanum nigtum polysaccarides on tumor growth in liver cancer loaded mice.Methods Established liver cancer loaded mouse model and divided randomly mice into five groups.Then calculated the tumor inhibitory rate and spleen index. We detected the expression of VEGF mRNA by in situ hybridization and Ki 67 protein by immune histochemistry.Results Low energy laser irradiation combined solanum nigtum polysaccharide could inhibit tumor growth and angiogenesis, the expression of VEGFmRNA, Ki 67 protein were decreased.Conclusion Low energy laser irradiation combined solanum nigtum polysaccharide can obviously inhibit the proliferation of liver cancer cells and angiogenesis ......