大鼠；肝性脑病；肝衰竭；病理____

大鼠急性与慢性肝衰竭肝性脑病模型对照研究

http://www.100md.com 《东南国防医药》 2006年第5期

大鼠；肝性脑病；肝衰竭；病理,,],大鼠；肝性脑病；肝衰竭；病理,1材料与方法,2结果,3讨论,参考文献

     [摘要] 目的制备SD大鼠的急、慢性肝衰竭(ALF、CLF)和肝性脑病(HE)动物模型。方法 SD大鼠100只，随机分为5组：正常对照组为Ⅰ号组，ALFⅡ、Ⅲ号组，CLF组Ⅳ、Ⅴ号组，每组20只。分别用5%硫代乙酰胺(TAA) 500mg/kg和3% TAA 300mg/kg灌胃，制作ALF组和CLF组肝性脑病动物模型。检测肝生化指标、血氨及肝脏和脑组织组织学对照分析。结果应用5%TAA 500mg/kg灌胃法建立的大鼠ALF及CLF实验模型，肝功能TBIL与3%TAA 300mg/kg组比较差异有非常显著性(P<001)。脑和肝脏组织病理学检查前者较后者更符合肝衰竭HE表现。死亡率和死亡时间两者相仿。结论 5%TAA 500mg/kg两次灌胃法制作大鼠肝衰竭方法简便易行，且更符合研究要求。

    [关键词] 大鼠；肝性脑病；肝衰竭；病理

    Control research of the models of hepatic encephalopathy in the big rat with acute and chronic liver failures

    SONG Minning1, SONG Yuna2, CHEN Fu3, LUO Meilan1 (1Infection Department,the 174th Hospital of PLA, Xiamen 361003,Fujian ,China；2Fuzhou Genenal Hospital of Nanjing Military Command,Fuzhou 350025,Fujian,China;3Xiamen University Xiamen,361005,Fujian,China)

    [Abstract] Objective To make SD big rat models of hepatic encephalopathy(HE) with acute and chronic liver failures(ALF/CLF) Methods 100 SD big rats were divided randomly into 5 group,each 20, the normal control groupⅠ, and ALFⅡ,Ⅲ and CLF Ⅳ,ⅤTwo different thioacetamide(TAA) dosage types of gastric lavage(5% 500mg/kg and 3% 300mg/kg) were set and the TBil, ALT/AST and blood ammonia were examined as well as analyzed on histopathologyResults The big rats ALF and CLF models which were built by 5% TAA 500 mg/ kg gastric lavage, TBil in quality of liver function was significantly different comparing with the group of 3%TAA 300 mg/ kg (P<001),whereas the hepatic and brain histopathology was more consistent to the features of LF HE in 500 mg/kg groupThe ratio and time of death was similar to each other Conclusion It is easier to make big rat ALF model by the 5% TAA 500mg/kg gastric lavage twice and also more according with the demands of research ......

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