[摘 要] 目的 对弥漫性大B细胞淋巴瘤(DLBCL)进行IgH基因重排研究。方法 用免疫组化法标记筛选DLBCL，用针对IgH 单轮扩增引物对DLBCL石蜡包埋组织进行多聚酶链反应(PCR)扩增检测克隆性基因重排。结果 60例DLBCL IgH基因重排阳性率为517%(31/60),其中中心母细胞型阳性率545%(30/55),免疫母细胞型阳性率00%(0/3),间变性大细胞型阳性率500%(1/2)。结论 DLBCL IgH基因重排检出率低于B细胞淋巴瘤总检出率, 可能与DLBCL 中存在IgH可变区体细胞超突变及背景细胞的数量有关。

    [关键词] 淋巴瘤；大细胞；弥漫性；基因重排；多聚酶链反应

    Detection of gene rearrangement of immunoglobulin heavy chain in diffuse large Bcell lymphoma

    CHEN Yu1,ZHAO Tong2,HAN XiQun2,WU BingXu1, CHEN MeiYan1 (1Pathology Department,the 95th Hospital of PLA, Putian 351100,Fujian ,China;2Pathology Department,Nanfang Medical University ,Guangzhou 510515,Guangdong,China)

    [Abstract] Objective To investigate the features of gene rearrangement of immunoglobulin heavy chain (IgH) in diffuse large Bcell lymphoma(DLBCL) Methods 60 cases of DLBCL were selected by immunohistochemical method and IgH clonal gene rearrangement were analysed by PCR from paraffinembeded sections derived from the cases above Results IgH gene rearrangements were detected in 517%(31/60) of DLBCL The positive rate of IgH gene rearrangement in centroblastic, immunoblastic, anaplastic large cell lymphoma was 545%,00% and 500% respectivelyConclusion The positive rate of IgH gene rearrangement in DLBCL is lower than that in total Bcell lymphoma,it may be correlated with somatic hypermutation in IgH variable region and the amount of background cells ...... 婵犵鈧啿鈧綊鎮樻径鎰畺闁靛ǹ鍎查煬顒€霉閿濆牊纭堕柡浣靛€濋獮宥団偓锝庡亝缁ㄦ氨绱掑☉姗嗘Ч缂佷緤濡囬幉鎾醇閻斿壊妲梺鍝勫暟婵數娑甸崼鏇炶摕闁靛／鍕暯闂佺懓鐡ㄩ悧鏃傜箔閸涱喗濮滈柦妯侯槸瀵潡鏌￠崒姘カ缂佽鲸绻堝畷锝夘敍濠靛棗骞嬮梺鍝勫閸ㄧ敻锝為敃鍌氱婵犲﹤鎳庨崑宥団偓娈垮枟濞叉﹢骞栭崨濠傜窞閺夊牃鏅滈崺鍌炴煃閵夛妇鐭庢繛鏉戞閹秵鎷呭ù瀣壕婵犲灚鎸剧粈澶愭偣閸ヮ剦妫戦柛瀣剁秮瀹曟瑧鎷犻懠顒佺秳濡ょ姷鍋炲ú妯恒€掗懜鐢碘枖閻庯綆鍋呴悡娆撴煟閵娿儱顏ら柍褜鍓氱划搴ㄥ极閹间焦鍤囬柟瀵稿Т椤ｅジ鏌嶉妷锔剧畺闁搞劊鍔戦崹鎯р堪閸愩劍鏋剧紓鍌氬暞閸ㄥ潡濡存径鎰伋婵犻潧饪撮崯蹇涙⒒閸屻倕鏋旈柍褜鍓ㄩ幏锟�


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