LIU Xianª²Li, WANG Yanª²Ming, WEI Liª²Min

    Department of General Surgery, First Affiliated Hospital, He¬ðnan University of Science and Technology, Luoyang 471003, China

    ¡¾Abstract¡¿ AIM: To probe into the protective influence on the mechanical and immune barriers exerted by recombinant human growth hormone (rhGH) at ileum in rats with obstructive jaundice. METHODS: In common bile duct (CBD)ª²ligated Wistar rats, morphological changes in ileac mucous membrane, sIgA variation in ileac juice and altered bacterial translocation were observed and compared before and after rhGH administration. RESULTS: CBDª²ligated rats had thinner ileac mucous membrane [(232¡À22) ¦Ìm vs (362¡À43) ¦Ìm] and shorter villi [(158¡À22) ¦Ìm vs (254¡À39) ¦Ìm]. Meanwhile, sIgA concentration in ileac juice dropped [(70¡À14) mg/L vs (162¡À32) mg/L] and bacterial translocation rate increased (3/12 vs 0/15). After using rhGH administration for 1 week, the ileac mucous membrane of CBDª²ligated rats get thicker [(308¡À34) ¦Ìm vs (232¡À22) ¦Ìm] and the villi get longer [(216¡À29) ¦Ìm vs (232¡À22) ¦Ìm] and stronger. Microstructure of ileac membrane get comparatively normalized. sIgA level in ileac juice rose [(114¡À18) mg/L vs (70¡À14) mg/L]. Rate of bacterial translocation decreased (2/15 vs 3/12). CONCLUSION: Rats with obstructive jaundice suffer from morphological and immune abnormalities in ileac membrane. rhGH administration can correct these abnormalities and afford a good protection on impaired mucosal and immune barriers in rats with obstructive jaundice. ......