Experimental study on apoptosis of ovarian cancer cells and reversal docetaxel drug resistance induced by Survivin antisense RNA

    MA Rong, CHEN Xiª²Hai, TANG Liª²Ping, GENG Xiaoª²Xing, ZHANG Yunª²Yan, ZHANG Yingª²Lei, WANG Jing

    1Department of Gynecology, Tumor Hospital, Harbin Medical University, Harbin 150040, China

    2Department of Surgery, Fourth Clinical College, Harbin Medical University, Harbin 150001, China

    3Heilongjiang Talent Center, Harbin 150001, China

    ¡¾Abstract¡¿ AIM: To establish Survivin antisense RNA and explore its effect on the apoptosis of ovarian cancer cells and the sensitivity to docetaxel and its mechanism of reversing docetaxel drug resistance. METHODS: Survivin antisense eukaryotic vector (antiª²pcDNA3ª²svv) was transferred into Skov3 cells by electroporation and positive clone was screened out. Survivin protein was detected by Western blot; effect of apoptosis inducement was observed by flow cytometry; sensitivity to docetaxel was examined by MTT; expression of MDRª²1 mRNA was detected. RESULTS: Survivin protein of positively cloned cells were reduced obviously as compared with that of nonª²transfered cells. Terminal apoptotic change was observed by flow cytometry and the apoptosis rate was 19%; IC50 of experimental group to docetaxel was (13.3¡À2.25) ng/mL and control group was (53.2¡À2.45) ng/mL, so the statistical difference was significant (P<0.01). Expression of MDRª²1 mRNA of Skov3ª²SVVanti group was reduced obviously as compared with that of Skov3 group (P<0.01). CONCLUSION: Survivin antisense RNA can induce ovarian cancer cell apoptosis and increase the sensitivity to docetaxel by reducing the expression of MDRª²1 mRNA. ......