轮状病毒；_VP6基因；基因_合成；_基因工程疫苗_

轮状病毒新型VP6基因的拼接合成和序列测定

http://www.100md.com 《第四军医大学学报》 2006年第19期

轮状病毒；,VP6基因；基因,合成；,基因工程疫苗,,轮状病毒；,VP6基因；基因，合成；,基因工程疫苗,0引言,1材料和方法,2结果,3讨论,【参考

     ZHANG CaiHong1,2, ZHANG QiangZhe1, ZHANG YanMing2

    1Life Science Platform, Centre of Chemical Genomics and Medicine Technology, Nankai University, Tianjin 300071, China, 2College of Animal Science and Technology, Northwest SciTech University of Agriculture and Forestry, Yangling 712100, China

    【Abstract】 AIM: To design and obtain novel rotavirus (RV) VP6 gene. METHODS: On the basis of our previous reports, RV VP6 gene was optimized through the gradual splicing method, according to the improved PCR strategy. RESULTS: The optimized cDNA sequence of RV VP6 gene was successfully synthesized, which was about 1220 bp and reached 55% in (G+C) content. CONCLUSION: The optimized RV VP6 gene was obviously increased about 20% in (G+C) content compared to wildtype RV VP6 gene. This study laid a further strong foundation for the development of novel oral rotavirus genetic engineering vaccine.

    【Keywords】 rotavirus; VP6 gene; genes, synthetic; genetic engineering vaccine

    【摘要】目的：设计获得轮状病毒(RV)新型VP6基因. 方法：本研究在前期工作的基础上 ......

百拇医药网 http://www.100md.com/html/DirDu/2006/11/14/29/38/41.htm

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