【摘要】 目的 对大肠埃希菌O157:H7的检测方法进行探讨，减轻日常工作量，节约成本，提高阳性检出率。方法 将PCR技术和免疫磁珠法相结合，样品先进行PCR检测，阳性的样品再以免疫磁珠法进行分离和鉴定。结果 从245件猪粪、牛粪样品中应用免疫磁珠和PCR结合方法，阳性检出率为8.98%，高于直接应用免疫磁珠法的阳性检出率6.12%。结论 此方法为大肠埃希菌O157:H7的检测提供了一个新的思路，节约了人力和物力，提高了工作效率和阳性检出率。

    【关键词】 免疫磁珠法；大肠埃希菌O157:H7；PCR技术

    Study on method for detecting E.coli O157:H7

    SHEN Li,XIE Xiao-hong,HAN Hua-zhong. Fengxian District Center for Disease Prevention and Control,Shanghai 201400,China

    【Abstract】 Objective To discuss the detection method for E.coli O157:H7.We can economize cost,release the work, enhance the positive rate.Methods Combined the polymerase chain reaction technique with immunomagnetic sand method,we could detect these samples with PCR at first, and then collected and detected the positive species by using immunomagnetic sand method.Results We have detected 245 species of dung by using this operational procedure. The result proved that the positive rate can be up to 8.98% above the rate of 6.12% by using immunomagnetic sand method only.Conclusion It is a new idea for us to release the work and enhance the positive rate by using this operational procedure for detect the E.coli O157:H7. ......