硫辛酸；脂多糖；肺损伤_急性；白细胞介素10；大鼠

硫辛酸对急性肺损伤大鼠IL10的影响

http://www.100md.com 《疑难病杂志》 2006年第5期

硫辛酸；脂多糖；肺损伤,急性；白细胞介素10；大鼠,,硫辛酸；脂多糖；肺损伤，急性；白细胞介素10；大鼠,1材料与方法,2结果,3讨论,参考文献

     【摘要】目的探讨硫辛酸(LA)对内毒素(脂多糖，LPS)诱导的急性肺损伤(ALI)大鼠白细胞介素(IL)10含量的影响。方法 72只大鼠随机分为4组，对照组、LPS组LPS+LA 30 mg干预组。LPS+LA 100 mg干预组。各实验组舌下静脉注射LPS诱导大鼠ALI模型，30 min后2个干预组分别给予LA 30 mg/kg和100 mg/kg舌下静脉注射，对照组给予等体积生理盐水。LPS注射1、3、6 h后测定血清及肺组织中IL10浓度。结果各实验组血清及肺组织中IL10分别在不同时间点显著高于对照组(P<0.01)。2个干预组血清及肺组织中IL10水平在3 h、6 h明显升高，均显著高于LPS组(P<0.01)，且LPS+LA 100 mg组显著高于LPS+LA 30 mg组(P<0.05)。结论 LPS可导致ALI的发生，LA可提高IL10水平，改善肺损伤。

    【关键词】硫辛酸；脂多糖；肺损伤，急性；白细胞介素10；大鼠

    The effects of lipoicacid on interleukin10 in acute lung injury rat

    BAI Jie，YU Zhanbiao，YANG Yongfeng，et al.

    Department of Emergency Medicine,the Affiliated Hospital of Hebei University，Baoding 071000,China

    【Abstract】 Objective To investigate the effects of lipoicacid (LA )on expression of interleukin10 (IL10) in rat with acute lung injury (ALI) induced by lipopolysaccharide(LPS).Methods 72 rats were randomly divided into four groups (normal group,LPS group, LPS+LA 30 mg group, LPS+LA 100 mg group).ALI model was made by hypoglossial injection of LPS,and 30 minutes later LA was injected in LPS+LA 30 mg group,and LPS+LA 100 mg group.At 1,3 and 6h after LPS injection, the lung homogenates and serum were prepared to detect IL10 levels.Results In LPS group, the levels of IL10 in serum and lung homogenates were higher than those of normal group (P<0.01),but lower than those of LPS+LA 30 mg group and LPS+LA 100 mg group at 3h and 6h after LPS injection (P<0.05).In LPS+LA 100 mg group,IL10 levels in serum and lung homogenates at 3h and 6h were higher than those of LPS+LA 30 mg group (P<0.05).Conclusion LPS could lead to ALI,LA could upregulate IL10 levels and improve the injury of lungs. ......

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