化学合成经修饰抗TIMP-2小干扰RNA对CCl4诱导肝纤维化动物模型的影响
http://www.100md.com
闻 颖, 马 力, 刘 沛
肝肾综合征;肿瘤坏死因子-α;肝素;1,4,5-三磷酸肌醇受体;离体灌注肾技术,化学合成经修饰抗TIMP-2小干扰RNA对CCl4诱导肝纤维化动物模型的影响,闻颖,马力,刘沛,闻颖,通讯作者
 |
| 第1页 |
参见附件(2089KB，5页)。
闻颖, 马力, 刘沛, 中国医科大学附属盛京医院传染科 辽宁省沈阳市 110004
闻颖, 2004年中国医科大学博士生, 主要从事肝肾综合征的研究.国家自然科学基金资助项目, No. 30270607
通讯作者: 刘沛, 110004, 沈阳市和平区三好街36号, 中国医科大学附属盛京医院传染科. syliupei2003@yahoo.com.cn
电话: 024-83956981 传真: 024-83956451
收稿日期: 2006-08-23 接受日期: 2006-09-21
Tumor necrosis factor-α enhances the expression of type 1 inositol 1,4,5-triphosphate receptors
Ying Wen, Li Ma, Pei Liu
Ying Wen, Li Ma, Pei Liu, Department of Infectious Diseases, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Supported by National Natural Science Foundation of China, No. 30270607
Correspondence to: Pei Liu, Department of Infectious Diseases, the Affiliated Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province,China. syliupei2003@yahoo.com.cn
Received: 2006-08-23 Accepted: 2006-09-21
Abstract
AIM: To clarify the mechanisms of renal vasoconstriction in hepatorenal syndrome (HRS) by investigating the effect of tumor necrosis actor-α (TNF-α) on the expression of type 1 inositol 1,4,5-triphosphate receptors (IP3R) in renal tissues.
METHODS: Ex vivo perfused rat kidney model was used in this study. Male Wistar rats were randomly divided into 3 groups: control group (group A), heparin (10 mg/L) treatment group (group B), and TNF-α (1 μg/L) treatment group (group C). After perfusion, immunohistochemical staining, Western blot, and real-time quantitative polymerase chain reaction (RTQ-PCR) were used to detect the distribution and expression of type 1 IP3R in renal tissues.
RESULTS: Immunohistochemical staining showed that type 1 IP3R protein was localized at the plasma region of glomerular mesangial cells and vascular smooth muscle cells in rat kidney, and the number of positive cells was significantly higher in group C than that in group A (U = 2 ......
您现在查看是摘要介绍页，详见PDF附件(2089KB，5页)。