胃癌组织中5-脂氧合酶的表达及其与磷酸化Akt的相关性
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李 勇, 李建英, 王小众, 邹来玉, 陈治新
胃肿瘤;5-脂氧合酶;磷酸化Akt;逆转录-聚合酶链反应;免疫印迹,李勇,李建英,王小众,邹来玉,陈治新,李勇,通讯作者:,电话:
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李勇, 李建英, 王小众, 邹来玉, 陈治新, 福建医科大学附属协和医院消化内科 福建省福州市 350001
李勇, 福建医科大学附属协和医院2004级硕士研究生.福建省科技人才创新基金资助项目, No. 2002J061
通讯作者: 李建英, 350001, 福建省福州市新权路29号, 福建医科大学附属协和医院消化内科. JYLi99@hotmail.com
电话: 0591-83357896-8482
收稿日期: 2006-07-08 接受日期: 2006-09-20
Expression of 5-lipoxygenase in gastric cancer and its correlation with phosphorylated Akt
Yong Li, Jian-Ying Li, Xiao-Zhong Wang, Lai-Yu Zou, Zhi-Xin Chen
Yong Li, Jian-Ying Li, Xiao-Zhong Wang, Lai-Yu Zou, Zhi-Xin Chen, Department of Gastroenterology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China
Supported by the Innovation Foundation for the Science and Technology Talents of Fujian Province, No. 2002J061
Correspondence to: Dr. Jian-Ying Li, Department of Gastroenterology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province,China. JYLi99@hotmail.com
Received: 2006-07-08 Accepted: 2006-09-20
Abstract
AIM: To investigate the expression of 5-lipoxygenase (5-LOX) in human gastric cancer and its effect on phosphorylated Akt (p-Akt).
METHODS: Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of 5-LOX mRNA, and Western blot was conducted to evaluate 5-LOX and p-Akt protein in 30 pairs of fresh gastric cancer and adjacent normal tissues.
RESULTS: In gastric cancer, the expression of 5-LOX was significantly higher than that in the adjacent normal tissues both at mRNA (76.7% vs 40%, P < 0.05) and protein (56.7% vs 30%, P < 0.05) level. The expression of p-Akt protein was also markedly higher in the cancer tissues than that in the adjacent normal ones (56.7% vs 26.7%, P < 0.05). There was no obvious correlation between the expression of 5-LOX and p-Akt protein(r = 0.186, P > 0.05).
CONCLUSION: The 5-LOX protein may promote the carcinogenesis and progression of gastric cancer, which is not correlated with PI3-K/Akt signaling pathway ......
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