胆囊收缩素；超氧化物歧化酶；NFκB；脂多糖类；胸主动脉；

CCK8对LPS诱导大鼠血管平滑肌细胞SOD基因表达及NFκB活性增高的影响

http://www.100md.com 《第四军医大学学报(新)》 2006年第20期

胆囊收缩素；超氧化物歧化酶；NFκB；脂多糖类；胸主动脉；肌,平滑,细胞学,,胆囊收缩素；超氧化物歧化酶；NFκB；脂多糖类；胸主动脉；肌，平滑,细胞学,CCK8对LPS诱导大鼠血管平滑肌

     MA LiQin, GU ZhenYong, DONG GuoKai, LI Na, GAO Feng, LIU Xia, MA ChunLing, CONG Bin, LING YiLing

    Department of Forensic Medicine and Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China

    【Abstract】 AIM: To investigate the effect of cholecystokinin octapeptide (CCK8) on SOD mRNA expression in rat thoracic aortic smooth muscle cells (TASMCs) induced by lipopolysaccharides (LPS) in vitro, and its mechanisms of signal transduction. METHODS: The TASMCs were isolated and cultured from rats and stimulated with LPS in the presence or absence of CCK8(10-10-10-6 mol/L) or vehicle alone or together. The expressions of Mn SOD and CuZn SOD mRNA were assayed by RTPCR; the IкBa protein level was detected by Western blot. The expression and localization of P65 protein, a subunit of NFκB, was assayed by immuocytochemistry. RESULTS: In rat TASMCs there were the basic expressions of Mn SOD and CuZn SOD mRNA. As compared with vehicle action, LPS induced the upregulation of Mn SOD and CuZn SOD mRNA, enhanced P65 protein expression in cytoblast (P<0.05) but reduced IκBa protein expression in cytoplasma (P<0.05). CCK8 in a dosedependent manner (P<0.05) obviously inhibited the abovementioned effects by LPS stimulation (P<0.05). CCK8 alone led to reduction in basic expressions of Mn SOD and CuZn SOD mRNA (P<0.05) but no change of P65 protein and IκBa protein expressions in TASMCs. CONCLUSION: CCK8 inhibits LPSinduced Mn SOD and CuZn SOD mRAN expressions by regulating NFκB activity in rat TASMCs. ......

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