再灌注损伤；肝功能；四肢；牛磺酸_

牛磺酸对大鼠肢体缺血再灌注后肝脏损伤的保护效应

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再灌注损伤；肝功能；四肢；牛磺酸,,再灌注损伤；肝功能；四肢；牛磺酸,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Protective effect of taurine against liver injury after limb ischemiareperfusion in rats

    WANG YinHuan, DONG ShuYun, ZHANG Na, ZHAO LiJun, LI HongJie, KONG XiaoYan, LIU LiHua, ZHANG LianYuan

    Department of Pathophysiology, North China Coal Medical College, Tangshan 063000, China

    【Abstract】 AIM: To observe the liver injury and investigate the protective effect of taurine on the liver function after limb ischemiareperfusion. METHODS: Thirty Wistar rats were randomly divided into control group，ischemiareperfusion(IR) group and taurine + ischemiareperfusion (TR) group (n=10 per group). The values of XOD, LDH, MDA, AST, ALT and SOD in plasma were respectively measured after 4 h limb ischemia and 4 h reperfusion in each group. XOD, MDA, ROS, MPO and [Ca2+] in the liver tissues and GSHPX, [Ca2+] in the liver mitochondria were observed. RESULTS: In IR group compared with the control group, the values of LDH [(190.16±13.36) μkat/L vs (122.39±14.87) μkat/L], XOD [(758.82±151.53) nkat/L vs (543.61±43.51) nkat/L], MDA [(5.19±0.67) nmol/L vs (1.27±0.21) nmol/L] , ALT [(78.40±6.45) nkat/L vs (20.50±3.70) nkat/L], AST [(47.70±4.47) nkat/L vs (25.25±2.98) nkat/L] in plasma increased, but SOD [(1.30±0.15) μkat/L vs (1.80±0.16) μkat/L ] reduced, XOD [(104.69±12.34) μkat/kg vs (59.01±10.50) μkat/kg], MDA [(2.66±0.08) nmol/L vs (1.29±0.14) nmol/L], ROS [(771.65±100.69) μkat/L vs (606.45±52.01) μkat/L], MPO (0.47±0.04 vs 0.28±0.06), [Ca2+][(0.248±0.050) mmol/L vs (0.123±0.014) mmol/L] in the liver tissues increased, GSHPx [(20.34±4.67) nkat/L vs (31.17±11.50) nkat/L] in the liver mitochondria were obviously reduced, but [Ca2+][(0.38±0.06) mmol/L vs (0.14±0.03) mmol/L] increased; In TR group compared with the IR group, the values of LDH [(158.29±4.87) μkat/L], XOD [(758.82±151.53) nkat/L], MDA [(2.81±0.19) nmol/L], ALT [(64.40±9.05) nkat/L], AST [(38.70±8.10) nkat/L] were reduced, but SOD [(1.50±0.17) μkat/L] in plasma of TR group increased; the values of XOD [(94.19±13.50) μkat/L], MDA [(1.67±0.12) nmol/L], ROS [(710.81±55.34) μkat/ L], MPO (0.36±0.04), [Ca2+][ (0.192±0.426) mmol/L] in the liver tissues decreased, the values of GSHPx[(22.50±3.17) nkat/L] in the liver mitochondria of TR group obviously increased, but [Ca2+] [(0.31±0.06) mmol/L] reduced. CONCLUSION: The taurine could protect the liver function of rats after limb ischemiareperfusion. ......

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