Killing effects of recombinant AAV expressing fusion peptide NT4p53(C22)Ant on human pancreatic cancer Miapacaª²¢òcells

    ZHOU Qi, JI Zongª²Zheng

    Research Laboratory of Medical Ultrasound, Second Affiliated Hospital, Medical College, Xi¬ðan Jiaotong University, Xi¬ðan 710004, China

    ¡¾Abstract¡¿ AIM: To study the killing effects of recombinant adenoª²associated virus(AAV) expressing fusion peptide NT4p53(C22)Ant on human pancreatic cancer Miapacaª²¢ò cells. METHODS: NT4p53(C22)Ant was constructed and subcloned into the vector of recombinant AAV, and that was amplified in 293 packaging cells. The recombinant plasmid AAVª²NT4p53(C22)Ant was transferred into Miapacaª²¢ò cells. The direct effects on human pancreatic cancer cell line Miapacaª²¢ò were detected with light microscope, MTT, flow cytometry and lactate dehydrogenase(LDH) release assay. RESULTS: It could be observed under microscope that the AAVª²NT4p53(C22)Ant could directly kill the Miapacaª²¢òcells. MTT revealed that the survival rate of the cells was decreased obviously. Flow cytometry showed that the number of apoptotic cells was significantly increased in AAVª² NT4p53(C22)Ant group as compared with control group and simple AAV group (P<0.05). LDH level went up a little (P>0.05). CONCLUSION: The AAVª²NT4p53(C22)Ant could cause the apoptosis of human pancreatic cancer Miapacaª²¢òcells to kill the cells directly. ......