遗传载体；HOGG1基因；克隆_分子；DNA修复_

人DNA修复基因HOGG1真核表达载体的构建及序列测定

http://www.100md.com 《第四军医大学学报》

遗传载体；HOGG1基因；克隆,分子；DNA修复,,遗传载体；HOGG1基因；克隆，分子；DNA修复,0引言,1材料和方法,2结果,3讨论

     Construction and sequencing of eukaryotic expression vector of human DNA base excision repair gene HOGG1

    WEI YongChang, NAN KeJun, HUI LingYun, HE DaLin

    Department of Medical Oncology, Research Center of Medical Laboratory, Department of Urinary Surgery, First Hospital, Xian Jiaotong University, Xian 710061, China

    【Abstract】 AIM: To clone human DNA base excision repair gene, 8oxoguanineDNA glycosylase (HOGG1), and construct its eukaryotic expression vector with sequence analysis. METHODS: Total RNA was extracted from the liver tissue of human foetus by the guanidinium thiocyanate method. The whole HOGG1 coding gene was amplified by RTPCR and cloned into eukaryotic expression vector pCMVMyc. Plasmid DNA was extracted and subjected to restriction enzyme analysis. Clones representing cDNA inserts were sequenced. RESULTS: The construction of eukaryotic expression vector pCMVMyc/ HOGG1 was successful and the resulted sequence completely coincided with the designs. CONCLUSION: The construction of eukaryotic expression vector pCMVMyc/ HOGG1 provides a new way for biological targeted therapy involving DNA base excision repair gene HOGG1. ......

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