乳腺肿瘤·脂氧合酶抑制剂·CD44V6·VEGF_

脂氧合酶抑制剂NDGA对乳腺癌MCF7细胞VEGF和CD44V6表达影响的研究

http://www.100md.com 《中国现代普通外科进展》 2006年第5期

乳腺肿瘤·脂氧合酶抑制剂·CD44V6·VEGF,,乳腺肿瘤·脂氧合酶抑制剂·CD44V6·VEGF,1材料和方

     【摘要】目的：探讨NDGA对乳腺癌MCF7细胞VEGF和CD44V6表达的影响。方法：将乳腺癌MCF7细胞系培养至对数生长期，分别以0、1、10、100μmol/L的NDGA处理48h后，RTPCR法检测细胞VEGFmRNA的表达水平，流式细胞仪检测细胞CD44V6的表达情况。结果：在1、10、100μmol/L的NDGA作用下，3组VEGFmRNA和CD44V6的表达显著低于对照组(F=2996.13,P<0.001;F=3435.08,P<0.001)。结论：NDGA明显下调MCF7细胞VEGFmRNA和CD44V6的表达，抑制肿瘤细胞的侵袭转移能力。

    【关键词】乳腺肿瘤·脂氧合酶抑制剂·CD44V6·VEGF

    The effect of lipoxygenase inhibitor (NDGA) on the expression of VEGF and CD44V6 in breast cancer MCF7 cells

    LIU Huantao,TANG Lubing,MA Rong

    Department of Breast Surgery,Qilu Hospital of Shandong University(Jinan 250012,China)

    【ABSTRACT】Objective:To explore the effects of VEGF and CD44V6 on breast cancer MCF7 cells by NDGA.Methods:MCF7 cells in the logarithmic growth state were selected and measured.There were four cell groups(concentration:0,1,10,100μmol/L)in the experiment.After 48h treatment of NDGA,the expression of VEGFmRNA was measured by RTPCR and the expression of CD44V6 was examined by flow cytometry.Results:After treatment of NDGA,the expressions of VEGFmRNA and CD44V6 in NDGA groups were significantly lower than that of the control group(F=2996.13,P<0.001;F=3435.08,P<0.001).Conclusion:NDGA can influence the invision and metastasis on MCF7 cells by means of the downregulated expression of VEGFmRNA and CD44V6. ......

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