针刺·灸法·乳腺肿瘤·细胞凋亡·细胞周期_

针灸血清”对乳腺癌MCF7细胞凋亡及细胞周期的影响

http://www.100md.com 《中国现代普通外科进展》 2006年第5期

针刺·灸法·乳腺肿瘤·细胞凋亡·细胞周期,,针刺·灸法·乳腺肿瘤·细胞凋亡·细胞周期,1材料与方法,2结果,3讨论,参考文献

     【摘要】目的：观察针灸对乳腺癌细胞凋亡及细胞周期的影响。方法：针灸刺激雌性Wistar大鼠双侧足三里、三阴交、内关穴，抽取大鼠“针灸血清”分别与MCF7细胞混合培养，分为电针组、艾灸组和对照组。MTT法检测细胞生长情况，72h后流式细胞技术检测细胞凋亡率及Fas表达率。结果：MCF7细胞Fas表达率明显增高，72h达57.70%；MCF7细胞生长受到明显抑制，12h、24h、72h抑制率分别达17.55%、24.71%和31.85%；作用72h后的细胞凋亡率分别为1.07%、8.72%、10.18%,与艾灸组相近，均明显高于对照组(P<0.05)。结论：“针灸血清”作用于MCF7细胞可能通过增加Fas的表达率来诱导细胞凋亡。

    【关键词】针刺·灸法·乳腺肿瘤·细胞凋亡·细胞周期

    The effect of acupunctural and moxibustional therapy on the apoptosis of breast cell MCF7

    LI Yan1,GUO Gang1,LI Bin2,SHI Xiufang3

    1Acupuncture and Moxibustion Department Qilu Hospital of Shandong University(Ji

    nan 250012,China)

    2Shandong Acdemy Institute of Traditional Chinese Medicine(Jinan 250014,China)

    3Hospital of Shandong Zouxian Powerplant(Zoucheng 273500,China)

    【ABSTRACT】Objective:To observe the effect on the apoptosis of breast cancer cell MCF7 with acupunctural and moxibustional therapy.Methods:Zusanli and Sanyinjiao neiguan in both sides of female Wistar mice were acupunctured and moxibusted.MCF7 cell was cultirated with the serum.To detecte the cytoxicity with MTT,and detecte the apoptosic rate and Fas expression rate with flow cytometry after 72 hours.Results:The Fas expression was obviously increased upto 57.70% after 72h.The proliferation of MCF7 was inhibited remarkably.The inhibition rate were 17.55%,24.71% and 31.85% after 12h,24h and 72h.The apoptosic rate were 8.72% and 10.18% after 72h,remarkably higher than the control group(P<0.05).Conclusion:Acupunctural and moxibustional serum could increase Fas expression rate to inhibit proliferation of MCF7 cell and induce its apoptosis. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/12/12/31/66/33.htm

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