表皮生长因子受体反义寡聚核苷酸抑制人结肠癌细胞的增殖
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曲 娴, 陈 杰
表皮生长因子受体;反义寡聚核苷酸;结肠癌细胞系;细胞增殖,曲娴,陈杰,通讯作者:,Inhibitoryeffectsofepidermal
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曲娴, 北华大学医学院生理学教研室 吉林省吉林市 132013
陈杰, 北京协和医院病理科 北京市 100074
通讯作者: 曲娴, 132013, 吉林省吉林市华山路3999号, 北华大学医学院生理学教研室. quxian6167@yahoo.com.cn
电话: 0432-4608410
收稿日期: 2006-09-25 接受日期: 2006-10-14
Inhibitory effects of epidermal growth factor receptor antisense oligonucleotide on the proliferation of human colonic cancer cells
Xian Qu, Jie Chen
Xian Qu,Department of Physiology, Medical College of Beihua University, Jilin 132013, Jilin Province, China
Jie Chen,Department of Pathology, Beijing Union Hospital, Beijing 100074, China
Correspondence to:Xian Qu, Department of Physiology, Medical College of Beihua University, 3999 Huashan Road, Jilin 132013, Jilin Province, China. quxian6167@yahoo.com.cn
Received:2006-09-25 Accepted:2006-10-14
Abstract
AIM: To construct a stable cell line through transfecting reverse transcription virus carrier with antisense epidermal growth factor receptor (AS-EGFR) into human colonic cancer cell line (LST174) and investigate its biological behavior.
METHODS: Reverse transcription virus carrier with AS-EGFR was transfected into human colonic cancer cell line LST174 by lipid mediating method. The cellular growth and transformation of malignant phenotype were detected by cellular counting, growth curve, MTT assay and rate of clone formation on soft agar.
RESULTS: The cells after stable transfection exfoliated easily with rough cell membrane and scattered piece-like growth. The growth of LST174/AS-EGFR cells was significantly as compared with that of the controls (optical density: 0 ......
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