Ad-p27mt转染重组腺病毒治疗裸鼠内人胃癌的分子机制
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朱金水, 张 强, 宋明全, 张卫平, 达 炜, 陈金联, 孙 群
胃癌;人突变基因p27;细胞凋亡朱金水,张强,宋明全,张卫平,达炜,陈金联,孙群.Ad-p27mt转染重组腺病毒治疗裸鼠内人胃癌的分子机制. 世界华人消化杂志2006;14(36)3477-3481,朱金水,张强,宋明全,张卫平
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朱金水, 张强, 宋明全, 张卫平, 达炜, 陈金联, 孙群, 上海交通大学附属第六人民医院消化科 上海市 200233
通讯作者: 朱金水，200233，上海市宜山路600号，上海交通大学附属第六人民医院消化科. zhujs1803@hotmail.com
电话: 021-64369181-8969 传真: 021-64837019
收稿日期: 2006-03-04 接受日期: 2006-03-29
Molecular mechanism of recombinant adenovirus Ad-p27mt therapy for human gastric cancer in nude mice
Jin-Shui Zhu, Qiang Zhang, Ming-Quan Song, Wei-Ping Zhang, Wei Da, Jin-Liang Chen, Qun Sun
Jin-Shui Zhu, Qiang Zhang, Ming-Quan Song, Wei-Ping Zhang, Wei Da, Jin-Liang Chen, Qun Sun, Department of Gastroenterology, the Six People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China
Correspondence to: Jin-Shui Zhu, Department of Gastroenterology, the Six People’s Hospital of Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, China. zhujs1803@hotmail.com
Received: 2006-03-04 Accepted: 2006-03-29
Abstract
AIM:To explore the effect of recombinant adenovirus Ad-p27mt on the apoptosis of human gastric cancer cells and its correlative mechanism.
METHODS:The recombinant adenovirus Ad-p27mt was constructed and then transfected into human gastric cancer cell line SGC-7901. The apoptotic peak of hypodiploid chromosomes in SGC-7901 cells was measured by flow cytometry, and the apoptosis of SGC-7901 cells was detected by TUNEL method.
RESULTS:Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18-h infection, the apoptotic peak of hypodiploids appeared before G1-S phase and the bands with apoptotic characteristics were shown by DNA electrophoresis. The apoptotic rate of SGC-7901 cells detected by TUNEL method was 89.4%±3.12% (Ad-p27mt group) and 3.12%±0.13% in Ad-p27mt-transfected cells and controls, respectively, and there existed significant difference between the two groups (P<0.01).
CONCLUSION: Human mutant p27 can induce the apoptosis of human gastric cancer cells in vitro ......
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