[摘 要\] 目的:测定维生素E霜中维生素E的含量。方法:采用反相高效液相色谱法，色谱柱：DiamonsilTM(钻石) C18柱，流动相：甲醇-氯仿(85∶15)，流速：1.0 ml·min-1 ，检测波长284 nm，柱温40 ℃。结果:维生素E的平均回收率为99.79%，方法精密度(RSD)为0.20%(n=6)。结论:该法可用于维生素E霜中维生素E的含量测定。

    [关键词\] 维生素E霜；维生素E；HPLC；含量测定

    Determination of Vitamin E in Vitamin E Cream by RP-HPLC

    LI Jian，LU Qi-wen，HE Lin.

    (Department of Pharmaceutics, Guangdong College of Pharmacy, Guangzhou,Guangdong 510006, China)

    Abstract: ObjectiveTo determine the content of Vitamin E in Vitamin E Cream. Methods RP-HPLC method was used. The chromatographic column was DiamonsilTM C18, the mobile phase was methanol∶chloroform (85∶15), the flow rate was 1.0ml·min-1,the detection wavelength was 284 nm, and the column temperature was 40 ℃. Results The average recoveries for Vitamin E was 99.79%, precision of the method was 0.20%(RSD, n=6). Conclusion The method could be used to determine the content of Vitamin E in Vitamin E Cream.

    Key words: Vitamin E Cream; Vitamin E; RP-HPLC; Assay

    维生素E是人的机体维持正常代谢和机能必需的脂溶性维生素 ......