摘要:目的 探讨慢性活动性乙型肝炎(CAHB)患者外周血活化抗原CD69、HLA-DR分子在T细胞表达的变化及其意义。 方法 以31例正常人作为对照，采用免疫荧光三色标记流式细胞术，检测30例CAHB患者外周血T细胞亚群及其CD69、HLA-DR抗原表达;同时用荧光定量PCR检测患者的病毒DNA载量。 结果 CAHB患者外周血CD3 + T细胞的百分率(63.78%±9.70%)与正常对照组比较差异有显著性(P<0.01)，CD4 + T细胞的百分率(45.88%±8.51%)明显低于正常对照组(P<0.001)，CD8 + T细胞的百分率(44.64%±7.10%)明显高于正常对照组(P<0.001);CD3 + T细胞CD69的表达(6.85%±2.83%)与正常人对照组比较差异也有显著性(P<0.01)，其中CD4 + T细胞CD69的表达(2.31%±0.94%)与正常对照组比较差异无显著(P>0.05)，CD8 + T细胞表达CD69水平(3.87%±1.81%)明显高于对照组(P<0.01);CD3 + T细胞上HLA-DR的表达(17.09%±4.23%)与正常对照组之间差异有显著性(P<0.001)，其中CD4 + T细胞中HLA-DR + 细胞(7.97%±2.43%)和CD8 + T细胞中HLA-DR + 细胞(5.52%±1.48%)均比正常对照组显著增高(P<0.01)(P<0.001);CD8 + CD69 + 、CD8 + HLA-DR + 细胞的数量与病毒DNA载量之间呈正相关。 结论 CAHB患者存在细胞免疫功能紊乱和淋巴细胞异常激活现象，T淋巴细胞的激活以CD8+T细胞为主，其在抗病毒感染中起着重要作用。

    关键词:慢性;乙型肝炎;T淋巴细胞;CD69;HLA-DR

    The expression of activated antigens in peripheral blood T lymphocyte subsets in patients with chronic active hepatitis B.

    ZHANG Ming-xia，LIN Li-hang，WANG Hui.

    (Department of Central Laboratory of Shenzhen Municipal Hospital of Chronic Diseases，Shenzhen518020，Guangdong，P.R.China)

    Abstract:Objective To investigate the expression of special marker for activation of peripheral blood T lymphocytes in patients with chronic hepatitis B virus and its significance. Methods Immunofluorescent three-color flow cytometry was used to study the expression of CD69and HLA-DR on T lymphocytes in30patients with chronic active hepatitis B virus and31normal controls.The viral load was determined by fluorescence quantitative PCR. Results Expression of CD3 + T cells were significantly lower in pa-tients(63.78%±9.70%)than that in controls(P<0.01) ......