[摘 要] 目的: 通过方法学试验对葡萄糖脱氢酶法测定血清葡萄糖方法进行评价。方法: 用已建立的方法进行重复性试验、回收试验、干扰试验、线性范围试验，并和HK法进行比较。 结果: 重复性试验批内 CV 值为1.33%，批间CV 值为1.42%，总 CV 值为2.01%;回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%，93.5%和91.4%，平均为96.6%;干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰;比较试验，与HK法比较其相关性为Y =1.038X-1.172，r =0.994;线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。 结论: 葡萄糖脱氢酶法是临床测定血清葡萄糖的较理想的方法。

    [关键词]方法学评价; 葡萄糖脱氢酶法; 葡萄糖

    Evaluation of glucose dehydrogenase method for determination of serum glucose

    ZHOU Li-ping，JIANG Xu-gan， XU-jun

    (1.School of Medical Technology， Jiangsu University， Zhenjiang Jiangsu，212001; 2.the Affiliated People′s Hospital of Jiangsu University，Zhenjiang Jiangsu， 212002，China)

    [Abstract] Objective: Glucose dehydrogenase(GDH) method for determination of serum glucose was e-valuated through methodology test. Methods: Iteration，recovery，interfere and linely test were tried by an established way，and also compared with HK method. Results: Within-run CV 、day-to-day CV and total CV were 1.33%、1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6%; anticoag-ulant、septic、uric acid and bilirubin under a certain concentration also did not interfere with the glucose re-sult in this method. Compared with HK method ......