Expression changes of LMO3 mRNA during mouse brain development

    HUI Ling, CAI Wenª²Qin, JI Hua, L¦a Tongª²De, YANG Jinª²Sheng, ZHAO Zhiª²Hua

    Experimental Center of Medicine, Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhuo 730050, China, Department of Neurobiology, Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China

    ¡¾Abstract¡¿ AIM: To examine the localization of neuronal specific transcription factor LMO3 mRNA in the development of central nervous system (CNS) of the mice. METHODS: Realª²time fluorescence quantitative RTª²PCR(FQ RTª²PCR) and in situ hybridization histochemical staining method with digoxinª²labeled cRNA probe were used to detect the expression of LMO3 mRNA. RESULTS: The FQ RTª²PCR showed that the expression of LMO3 mRNA was the highest during pregnancy, then it decreased significantly after birth and almost remained the same level until adult. LMO3 mRNA expression was extensive in brain at early embryonic stages (E10.5ª²11.5 d), but not detected at other parts of embryo. The high level in brain was still seen over midª²(E15.5d) and lateª² (E17.5ª²P0 d) embryonic brain and showed less different signal. During postnatal development, LMO3 mRNA was found gradually regionalized and showed the difference of signal in different parts of brain. LMO3 mRNA expression of P14ª²P60 d was still extensive although the extent was gradually more regional than that of P7d. The signal of LMO3 mRNA was mainly located in different nucleus clony and showed different intensity. CONCLUSION: LMO3 plays a role in brain development during embryonic period and postnatal differentiation and specification of specific brain regions. ......