Comparison of antiviral effect between Xª²region and Pª²region antisense RNA in HBV transgenic mice

    ZHAO Wei, CHEN Hong

    Department of Infectious Diseases, First Hospital, Lanzhou University, Lanzhou 730000, China

    ¡¾Abstract¡¿ AIM: To investigate the effect of antisense RNA of hepatitis B virus (HBV) in X region and P region on replication and expression of HBV gene in HBV transgenic mice. METHODS: Recombinant vector plasmids expressing antisense RNA complementary to HBV X region and antisense RNA complementary to HBV P region were constructed and injected into transgenic mice through tail vein. The experimental mice were divided into blank, PLXSN, PLXSNª²asX and PLXSNª²asP groups. HBV DNA in serum was tested by fluorescent quantitative PCR and Nestedª²PCR. RESULTS: The expression of HBV DNA in serum was inhibited in PLXSNª²asX group at day 2 after injection and still inhibited at week 8, while it was inhibited in PLXSNª²asP group at week 4 and reached the lowest at week 8. In PLXSNª²asX group and PLXSNª²asP group, the strongest inhibitory effect on the expression of HBV DNA reached 58% at the 2nd week and 8th week after treatment respectively. The serum HBV DNA of 2/8 mice in the PLXSNª²asX group and 1/8 mice in the PLXSNª²asP group became negative.CONCLUSION: The antiviral effect of antisense RNA in X region of HBV was earlier than that of antisense RNA in P region of HBV. But the difference of antiviral effect between the 2 regions was not significant. ......