【摘 要】 目的 观察蚕丝对前脂肪细胞吸附作用及蚕丝对前脂肪细胞形态和功能的影响，为脂肪组织工程支架选择提供依据。 方法 从家蚕蚕茧中抽丝所得的原料蚕丝，经胰蛋白酶消化后与原料蚕丝分别制成三维支架，将前脂肪细胞制成细胞悬液接种在支架上，倒置显微镜和扫描电镜观察前脂肪细胞的吸附和生长。 结果 在消化后的蚕丝三维支架可看见前脂肪细胞1～2d后完全贴壁。培养3～7d细胞生长增殖十分活跃，两周时，蚕丝支架网眼充满前脂肪细胞，扫描电镜见细胞与支架紧密贴附适度伸展并有基质分泌。 结论 蚕丝对前脂肪细胞具有良好的吸附作用，并能维持前脂肪细胞正常形态和功能，蚕丝是前脂肪细胞立体培养时的天然支架。

    【关键词】 3T3-L1前脂肪细胞;细胞培养;蚕丝;组织工程

    The experimental investigation of preadipocyte cultured on scaffolds of silks in vitro XIE Li-na，LIUXiu-hua.(De-partment of Oral Surgery，The Second Affiliated Hospital of Jinzhou Medical College，Liaoning121001，China)

    【Abstract】 Objective To observe the effects of silks on attachment，shape and function of preadipocyte cells cul-tured in vitro.Methods The silks from silk-worm cocoons were digested by trypsin and formed three-dimensional for preadipocyte cell.The cells were inoculated on silk scaffolds and cultured.The growth and function of preadipocyte cell were observed and measured with inverted microscopy and scanning electronic microscopy.Results Predipocyte cells were fixed on digested silks wall1or2days later ......