尼莫地平对生长受限胎鼠脑组织形态学及神经细胞凋亡率的影响
尼莫地平；胎儿生长受限；神经系统损伤,,尼莫地平；胎儿生长受限；神经系统损伤；凋亡,1材料与方法,2结果,3讨论,参考文献：
摘要：目的 探讨生长受限胎鼠脑组织神经细胞凋亡率变化，以及孕期应用钙离子拮抗剂尼莫地平干预对神经系统损伤是否具有保护作用。方法 SD孕鼠随机分为3组：①组Ⅰ：行假手术；②组Ⅱ，采用缩窄子宫动脉法建立FGR模型；③组Ⅲ：建模方法同组Ⅱ，术后予尼莫地平治疗。电子天平对新生仔鼠脑组织称重，比较各组仔鼠脑重。脑组织切片，HE染色，观察脑组织形态学变化。通过TUNEL法检测各组新生仔鼠皮层和海马CA1区神经细胞凋亡。结果 ①组Ⅰ仔鼠脑重为0.227±0.168g，明显高于组Ⅱ(0.208±0.192g，P<0.01)以及组Ⅲ(0.215±0.139g，P<0.01)，而Ⅱ、Ⅲ两组之间差异无统计学意义(P>0.05)。组Ⅱ部分仔鼠脑组织皮层变薄，存在出血、水肿灶，而组Ⅰ和组Ⅲ仔鼠未见明显变化。②组Ⅲ仔鼠皮层神经细胞凋亡率显著低于组Ⅱ(分别为2.98±0.54个/HP 和5.05±1.17个/HP, P<0.01)，但高于组Ⅰ(2.3±0.69个/HP，P<0.01)。③组Ⅲ仔鼠海马CA1区神经元凋亡率中位数为0.83个/HP，低于组Ⅱ(1.50个/HP，P<0.01)，高于组Ⅰ(0.50个/HP，P<0.05)。结论 钙离子拮抗剂尼莫地平孕期治疗对生长受限仔鼠神经系统损伤有保护作用。关键词：尼莫地平；胎儿生长受限；神经系统损伤；凋亡
Effect of Nimodipine Treatment During Gestation on Cerebral Histomorphology and Neuron Apoptosis of Intrauterine Growth Restricted Rat Fetus
WANG Yinglan, ZHANG Jianping, YANG Dongzi,et al
(Department of Obstetrics and Gynecology, The Second Afficiated
Hospital, Jinan University, Shenzhen Guangdong 518020, China)
ABSTRACT:Objective To study the properties of cerebral histomorphology and neuron apoptosis in growth restricted rat fetus, and whether nimodipine treatment during gestation can attenuate nervous system injuries. Methods Pregnant SD rats were randomly divided into 3 groups. Sham operation was exerted for rats in group Ⅰ, while bilateral uterine arteries were partially ligated in group Ⅱ and Ⅲ. Nimodipine (0.2mg/kg/d) was administrated subcutaneously for the group Ⅲ. Each fetus whole brain was weighted by electronic balance. Cerebral morphological changes were examined by light microscopy. Neuron apoptosis in cortex and hippocampus was detected by TUNEL. Results ①The brain weight of group Ⅰ (0.227±0.168g) was significantly higher than group Ⅱ(0.208±0.192g ......
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