摘要:目的 建立痤疮洗剂有效成份检测方法。 方法 采用薄层色谱法对处方中的黄柏和大黄进行定性鉴别;用薄层扫描法测定黄柏中盐酸小檗碱的含量。 结果 薄层图谱斑点清晰，阴性对照无干扰。盐酸小檗碱进样量在0.04μg～0.20μg范围内线性关系良好(r=0.9950)，平均加样回收率为98.69%，RSD=1.66%。 结论 本方法易于操作，结果准确，重现性好。

    关键词:痤疮洗剂;盐酸小檗碱;薄层扫描

    The detection of active component of Berberine hydrochloride in Acne Lotion.

    PENG Jian-mei，PENG Xiao-yun，XIE Qiao-e，et al.

    (Dongguan Municipal chronic Disease Hospital，Dongguan523008，Guangodng，P.R.China)

    Abstract:Objective To establish a method for detection of active component in acne lotion. Methods The scutell aria and phellodendron were identified by thin layer chromatography and the content of berberine hydrochloride was determined by thin layer chromatography scanning. Results The chromatogram spots were clear and no interference was found in the negative reference.The linear range of berberine hydrochloride on calibration curve was between0.04μg and0.20μg(r=0.9950).The average recovery rate was98.69%and RSD=1.66%. Conclusion This preparation method was easy to manipulate and the qualitywas stable and controllable as well as reproducible. ......