西咪替丁对胃癌细胞SGC-7901增殖及凋亡的影响
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姜成钢, 李嘉斌, 徐惠绵, 于 淼, 吴 涛, 刘芙蓉
胃肿瘤;西咪替丁;凋亡;细胞周期,姜成钢,徐惠绵,于淼,吴涛,李嘉斌,刘芙蓉,姜成钢,通讯作者:,电话:,传真:,收稿日期:,接受日期:,Effectofcimetidineontheproliferationandapoptos
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姜成钢, 徐惠绵, 于淼, 吴涛, 中国医科大学附属第一医院肿瘤外科 辽宁省沈阳市 110001
李嘉斌, 刘芙蓉, 中国医科大学细胞生物学教研室 辽宁省沈阳市 110001
姜成钢, 医学硕士, 医师, 主要从事胃肠道肿瘤基础及临床研究.
通讯作者: 徐惠绵, 110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院肿瘤外科. huimianxu@163.com
电话: 024-83283555 传真: 024-22703578
收稿日期: 2006-08-08 接受日期: 2006-11-02
Effect of cimetidine on the proliferation and apoptosis of human gastric cancer cell line SGC-7901
Cheng-Gang Jiang, Jia-Bin Li, Hui-Mian Xu, Miao Yu, Tao Wu, Fu-Rong Liu
Cheng-Gang Jiang, Hui-Mian Xu, Miao Yu, Tao Wu, Department of Oncology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Jia-Bin Li, Fu-Rong Liu, Department of Cell Biology, China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Hui-Mian Xu, Department of Oncology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province,China. huimianxu@163.com
Received: 2006-08-08 Accepted: 2006-11-02
AbstractAIM: To investigate the effect of cimetidine on the proliferation and apoptosis of human gastric cancer cell line SGC-7901 and its related mechanism.
METHODS: Human gastric cancer cell SGC-7901 was cultivated by routine method, then treated with different concentrations of cimetidine. The proliferation of SGC-7901 cells was examined by MTT assay, and the cell cycle and apoptosis were detected by flow cytometry. After Hoechst33258 staining, the morphologic changes of SGC-7901 cells were observed under fluorescence microscope, and the ultrastructure of the cells was investigated by transmission electron microscopy. The levels of Bcl-2 and Bax protein expression were detected by Western blot analysis.
RESULTS: After dealing with cimetidine (0.5, 1, 2.5, 5, 10 mmol/L) for 24 and 48 hours, we found that cimetidine significantly inhibited the proliferation of SGC-7901 cells in a time- and concentration-dependent manner (24 h: 0 ......
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