紫杉醇冻干脂质体的制备和稳定性的初步考察
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张 蓓，王东凯
(沈阳药科大学 药学院 辽宁 沈阳 110016)
摘要：目的 制备紫杉醇脂质体和紫杉醇冻干脂质体，并对其冻干前后的性质进行比较。 方法 采用薄膜分散法制备紫杉醇脂质体，用冷冻干燥技术将其冻干并进行形态和粒径的比较。采用质量分数5 % 的TritonX-100乙醇溶液对冻干前后的紫杉醇脂质体进行破乳测定含量，用Sephadex G- 50分离紫杉醇脂质体和游离药物。 结果 冻干前紫杉醇脂质体的平均粒径为210 nm，冻干后脂质体的平均粒径为279 nm。冻干前后脂质体的含量和包封率变化不大，离心加速试验的结果也没有明显区别。 结论 采用薄膜分散-超声法能够制备出包封率较高、粒径比较均匀的脂质体，经冷冻干燥后，其各项性质没有明显的变化。
关键词：药剂学；脂质体；薄膜分散法；紫杉醇
中图分类号：R94 文献标识码：A
contents
Preparation of paclitaxel liposome and study on its stability
ZHANG Bei, WANG Dong-kai
(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)
Abstract：Objective To preparation paclitaxel liposome and proliposome and compare their differences. Methods The paclitaxel liposome was prepared by film-sonication method and freeze-drying technique was used to prepare the proliposome. 5 % TritonX-100 ethanol solution was used to destroy the liposome for determination of paclitaxel content. Sephadex G-50 was used to separate the liposome and the free paclitaxel. Results The mean size of the paclitaxel liposome and proliposome were 210 nm and 279 nm, respectively. There are slight differences of content ,the entrapment efficiency and accelerated test between liposome and proliposome. Conclusions Using film-sonication method can prepare liposome with high entrapment efficiency and uniform size distribution.There are no obvious difference between liposome and proliposome.
Key words: pharmacuetics；liposome；film-dispersion method；pacbi taxol
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