Differentially expression of genes associated with cytoskeletal remodeling of peripheral blood eosinophils during asthmatic attack

    CAI Shaoª²Xi, ZHAO Haiª²Jin, DING Yanª²Qing, LI Wenª²Jun, ZHOU Bin, XIAO Jun

    Department of Respiratory Diseases, Nanfang Hospital, Department of Pathology, School of Basic Medicine, School of Public Health and Tropical Medicine, Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

    ¡¾Abstract¡¿ AIM: To determine the differentially expressed genes associated with cytoskeletal remodeling of peripheral blood eosinophils(EOS) in patients during asthmatic attack. METHODS: Doubleª²stranded cDNA was amplified from total RNA of EOS at the time of asthmatic attack and after improvement respectively by Super SMART cDNA Synthesis techology, Suppression subtractive hybridization(SSH) and PCRª² select differential screening technology were used to detect differentially expressed genes and then differentially expressed genes were sequenced. Homology analysis was conducted by comparing these gene sequences based on GenBank database. RESULTS: Highª²efficiently upª²regulated and downª²regulated subtractive cDNA libraries were constructed successfully; differential screening and homology analysis identified 6 differentially expressed genes associated with cytoskeletal remodeling, including slingshot 2L(SSHª²2L), PP1 catalytic subunit, beta isoform(PP1C¦Â), dedicator of cytokinesis 8(DOCKª²8), Homo sapiens protein tyrosine phosphatase nonª²receptor type 6 and 12(PTPN6, PTPN12), myosin regulatory light chain interacting protein(MIR). CONCLUSION: The differentially expressed genes associated with cytoskeletal remodeling may participate in exacerbation of asthma. Further study on the genes¬ð function may provide new gene target for asthma treatment. ......