牙髓培养细胞体外诱导矿化能力：冠髓与根髓的比较 (pdf)

    【摘要】 目的 比较人牙髓的根、冠髓培养细胞体外诱导矿化能力，探讨牙髓干细胞在牙髓中的定位。方法 用组织块法对人牙的根髓和冠髓细胞进行原代和传代培养，将第五代培养细胞用条件培养液(20%DMEM+10nmol/L地塞米松+10mmol/L β-甘油酸钠+50mg/L L-抗坏血酸)诱导矿化，在诱导后第10、20、30天对细胞进行Von Kossa钙染色。结果 根髓中形成的矿化小结比冠髓中更早、更强烈。结论 根髓比冠髓更容易诱导矿化。牙髓干细胞可能存在于根髓和冠髓之中，在根髓中比冠髓中具有更高的密度。

    【关键词】 牙髓干细胞；细胞培养；矿化结节

    The induced mineralization ability in cultured dental pulp cells:the comparation between coronal and root pulp cells

    YANG Qing-ai,WANG Xiu-yin, WANG Jian-hua,et al.Shandong Xiehe Vocational Technology College,Jinan 250100， China

    【Abstract】 Objective To compare the mineralization ability between cultured dental root and coronal pulp cells,and to investigate the localization of dental pulp stem cells(DPSCs) in dental pulps. Methods The human dental root and coronal pulp cells were cultured in tissue-explant method,the 5thgeneration of cells were subcultured with conditioning medium(20%DMEM+10nmol/L +dexamethasone+50mg/L ascorbic acid) to induce mineralization. At the days of 10,20,30 after inducing,von Kossa for calcium staining was used to investigate the mineralization ability in root and coronal pulp cells. Results The mineralization nodules appeared earlier and stronger in the root pulp cells than that in the coronal pulp cells observed in phase-contrast microscope and von Kossa staining. Conclusion Root dental pulp cells could be induced to mineralization more easily than the coronal pulp cells . DPSCs may exist in both root and coronal pulp, and its density in the root pulp is higher than that in coronal pulp. ......