应激；糖皮质激素；星形细胞；钙_

糖皮质激素致星形胶质细胞内钙浓度的快速升高

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应激；糖皮质激素；星形细胞；钙,,应激；糖皮质激素；星形细胞；钙,1材料和方法,2结果,3讨论

     Rapid elevation of intracellular free calcium concentration in astrocytes after treated by glucocorticoid

    LI MaoQuan1,2, WANG YanYan3, YANG Shu1, CHEN ZhaoQiong1, YANG XiaoHong1, YU ZhengPing2

    1Department of Public Health, Chengdu Medical College, Chengdu 610083, China,2Department of Occupational Health, 3Department of Medical Genetics, Third Military Medical University, Chongqing 400038, China

    【Abstract】 AIM: To explore the mechanisms of glucocorticoid (GC) leading to the elevation of intracellular free calcium concentration ([Ca2+]i) in astrocytes in early effect phase. METHODS: A cell culture model of neonatal Wistar rat hippocampal astrocytes was used. After exposed to corticosterone (CORT) (1 μmol/L), NmethylDaspartate (NMDA), MK801 and BSACORT, respectively, changes of [Ca2+]i in astrocytes were observed by laser confocal microscope. RESULTS: CORT induced rapid elevation of intracellular Ca2+ fluorescence intensity within 10 min (177.1±3.8， P<0.01) compared with controls (141.2±4.7), but induced no significant change when there was no Ca2+ out of the cell (148.5±6.3). NMDA caused further increase of intracellular Ca2+ after pretreatment with CORT (195.3±7.2 ......

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