Design and analysis of linkers among secretary singleª²chain bifunctional antibody fusion molecules with bioinformatics software

    LI Yingª²Tao, WU Xingª²An, QIU Liª²Jun

    Department of Medical Electronic Engineering, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: AIM: To analyze the secondary structure and the physical and chemical characteristics of the secretary antiª²osteosarcoma singleª²chain bifunctional antibody fusion protein with bioinformatics software. METHODS: The sites of enzyme were selected for the sequence of linker. Bioinformatics software was used to analyze structural change of the singleª²chain antibody fragments of variable domainª²tumor necrosis factor (ScFvª²TNF) fusion protein, and then DNAssist and ANTHEPROT V5 were used to analyze its sequence, secondary structure, and physical and chemical characteristics. RESULTS: On the fusion genes, the sites of enzyme were selected for the sequence of linker, and the secondary structure of the fusion protein was obtained with DNAssist, and the physical and chemical characteristics of the fusion protein were forecasted with ANTHEPROT V5. CONCLUSION: The software for bioinformatics should be fully utilized to analyze biological information, and be improved continuously. Bioinformatics can shorten the course of pharmaceutical development, at the same time can help us to explore the geneª²based medicine. ......