冬凌草；_总黄酮；_正交实验_____冬凌草；

冬凌草中总黄酮提取工艺研究与含量测定

http://www.100md.com 《时珍国医国药》 2006年第10期

冬凌草；,总黄酮；,正交实验,,冬凌草；,总黄酮；,正交实验,1仪器与材料,2方法与结果,3讨论,参考文献：

     摘要：目的研究冬凌草中总黄酮的提取工艺并测定总黄酮的含量。方法采用正交实验进行提取工艺的优选。紫外分光光度法测定总黄酮的含量。结果采用最佳提取工艺测定了不同部位不同产地的冬凌草中总黄酮含量，花中总黄酮含量最高，不同产地含量差别比较大。结论该方法简单、可靠、稳定、重现性好。

    关键词：冬凌草；总黄酮；正交实验

    Extracting Technology and Determination of the Total Flavonoid of Rabdosia rubescens (Hemsl.)Hara

    QU Hongli , KE Yu , YIN Peng ,LIU Hongmin*

    (New Drug Research & Development Center, Zhengzhou University, Zhengzhou 450052 ,China)

    Abstract：ObjectiveTo study the extracting technology and content of flavonoids of Rabdosia rubescens (Hemsl.) Hara. MethodsThe content of total flavonoid was determined with ultraviolet spectrophotometry at 504nm. ResultsThe content of total flavonoid differs among different sources and is the highest in flower. ConclusionThe method is simple, reasonable and stable.

    Key words：Rabdosia rubescens(Hemsl.) Hara; Total flavonoid; Orthogonal experiment

    冬凌草Rabdosia rubescens (Hemsl.)Hara ......

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