丹参单体对TNF-α诱导大鼠动脉平滑肌细胞MCP-1和IL-1β的影响 (pdf)

    【摘要】 目的 观察丹酚酸B和丹参酮ⅡA对肿瘤坏死因子(TNF-α)诱导的血管平滑肌细胞(SMC)白细胞介素-1β(IL-1β)和单核细胞趋化蛋白-1(MCP-1)的影响，探讨丹参单体抗动脉粥样硬化(AS)的作用机制。方法 体外培养大鼠主动脉SMC；RT-PCR法检测SMC MCP-1 mRNA的表达；放免法检测SMC培养上清IL-1β的水平。结果 TNF-α促进SMC MCP-1mRNA的表达，提高IL-1β水平(P值均<0.01)。两种丹参单体均抑制上述因子的表达，其中，对于抑制MCP-1mRNA的表达以丹参酮ⅡA效果更佳。结论 丹参单体抗AS作用的机制之一是抑制炎症相关因子的表达，丹参酮ⅡA抗炎效果优于丹酚酸B。

    【关键词】 肿瘤坏死因子；单核细胞趋化蛋白1； 白细胞介素-1β；丹酚酸 B；丹参酮ⅡA

    Effect of salvia miltiorrhiza monomer on expression of monocyte chemotactic protein-1 and interleukin-1β in cultured rat vascular smooth muscle cells induced by tumor necrosis factor α

    JIANG Xi-juan, ZHAO Gui-feng, LU Bin,et al. Department of Pathology， Tianjin University of Traditional Chinese Medicine， Tianjin 300192， China

    【Abstract】 Objective To observe the effect of salvianolic acid B and tanshinone ⅡA on monocyte chemotactic protein-1 and interleukin-1β in cultured smooth muscle cells induced by TNF-α and to investigate the molecular mechanism of salvia miltiorrhiza monomer against atherosclerosis.Methods Rat aortic smooth muscle cells (SMC) were cultured ......