半夏厚朴泡腾片；_厚朴酚；_和厚朴酚；_高效液相色谱

高效液相色谱法测定半夏厚朴泡腾片中厚朴酚及和厚朴酚的含量

http://www.100md.com 《时珍国医国药》 2006年第11期

半夏厚朴泡腾片；,厚朴酚；,和厚朴酚；,高效液相色谱,,半夏厚朴泡腾片；,厚朴酚；,和厚朴酚；,高效液相色谱,1仪器与试药,2方法与结果[2,3],3讨论,参考文献：

     摘要：目的建立半夏厚朴泡腾片中厚朴酚、和厚朴酚含量的测定方法。方法以依利特C18(4.6 mm × 200 mm,5 μm) 色谱柱分离厚朴酚、和厚朴酚，流动相甲醇水乙腈(31.5∶38.5∶30.0)，检测波长294 nm。结果线性范围：厚朴酚0.54～13.61 μg，r＝0.999 7(n＝7)，和厚朴酚0.11～2.82 μg，r＝0.999 7(n＝7)。厚朴酚平均回收率100.92%，SRD＝1.79%(n＝6)，和厚朴酚平均回收率101.23%，RSD＝1.79%(n＝6)。结论该方法简便易行，稳定性较好。

    关键词：半夏厚朴泡腾片；厚朴酚；和厚朴酚；高效液相色谱

    Determination of the Content of Magnolol and Honokiol in BanxiaHoupu Effervescent Tablets by HPLC

    ZHENG Ping，WANG Wenzhong，OU Yingyong，ZHANG Guanghou，LI Ting，GUO Wei

    (Department of Pharmaceutical Engineering，School of Biotechonology & Food Science，Tianjin College of Commerce，Tianjin 300134，China)

    Abstract：ObjectiveTo establish a method for determining the content of magnolol and honokiol in BanxiaHoupu effervescent tablets.MethodsMagnolol and honokiol were separated with Elite C18column(4.6 mm × 200 mm,5μm) and detected at 294 nm, using methanolacetonitrilewater(31.5∶38.5∶30.0)as mobile phase.ResultsThe standard curves for magnolol and honokiol were linear in the range of 0.54～13.61μg (r＝0.999 7) and 0.11～2.82 μg (r＝0.999 7). The average recoveries of magnolol and honokiol were 100.92% (RSD＝1.79%) and 101.23% (RSD＝1.79%). ConclusionThe method is simple and quick, which is suitable for practical use. ......

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