转化生长因子β1；_Burkitt淋巴瘤；

转化生长因子β1对淋巴瘤细胞生长及cmyc基因表达的影响

http://www.100md.com 《时珍国医国药》 2006年第9期

转化生长因子β1；,Burkitt淋巴瘤；,cmyc基因,,转化生长因子β1；,Burkitt淋巴瘤；,cmyc基因,1材料和方法,2结果,3讨论,参考文献：

     摘要：目的利用Burkitt淋巴瘤细胞株Jiyoye细胞探讨转化生长因子β1(TGFβ1)对淋巴瘤细胞生长的抑制作用和cmyc 基因的影响。方法利用MTT及RTPCR方法检测细胞生存率、cmyc 基因的表达水平。结果不同浓度的TGFβ1作用于Jiyoye细胞24 ，48 h和72 h后，细胞生存率随浓度的升高和时间的延长而逐渐降低。实验组各时间段的生存率与对照组相比显著降低(P<0.01)，同时，实验组各组间均有显著性差异(P<0.01)。实验组中cmyc 基因的表达24 h开始下降，48 h和72 h时明显受抑制，与对照组相比有显著性差异(P<0.01)。结论 TGFβ1可在浓度和时间依赖性抑制Jiyoye细胞株生长的同时抑制cmyc基因的表达。

    关键词：转化生长因子β1； Burkitt淋巴瘤； cmyc基因

    Effect of Transforming Growth Factor β1 on Cell Growth and cmyc Gene Expression on Lymphoma Cell Line

    LI Zhuhu， LIN Zhenhua， LEE Mija

    (Department of Pathology, College of Medicine, Yanbian University, Yanji 133000, China;Department of Pathology, College of Medicine, Chosun University Gwoungju, South Korea)

    Abstract：ObjectiveTo investigate the inhibition of transforming growth factorbeta1 (TGFb1) on cell growth and cmyc gene expression on Burkitt lymphoma cell lineJiyoye.MethodsThe cell survival rate and cmyc gene expression were detected by using MTT assay and RTPCR.ResultsAfter 24, 48 and 72 h of different concentration of TGF β1 action on Jiyoye cell line, the cell survival rate decreased along with the increase of concentration and extension of the time. Compared with the control group, the cell survival rate of every stage in experimental group significantly decreased(P<0.01). At the same time, there was also significant difference among the experimental groups(P<0.01). Cmyc gene expression began to reduce at 24 h, and significantly inhibited at 48 h and 72 h ......

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