【摘要】 目的 考察灯盏细辛中黄酮类成分(总黄酮、灯盏花素)对体外高压诱导的视网膜神经节细胞(retinal gan-glion cells，RGCs)凋亡的影响，探讨其视神经保护作用的物质基础。 方法 采用胰酶消化法将24只出生2～3d的SD(Sprague-Dawley)乳鼠视网膜制成细胞悬液，接种于经多聚鸟氨酸(HA)和层粘连蛋白(LN)包被的盖玻片中。培养72h后，将覆有细胞的血盖片转入加压装置中，加入黄酮类成分，继续培养48h，采用Fas蛋白免疫组化染色法及原位末端脱氧核苷酸转移酶标记(Tunel-POD)法进行检测，每天观察细胞形态，同时对部分细胞行NSE染色检查。 结果 细胞生长良好，NSE染色表明，85%以上的细胞为RGCs。给药组的Fas蛋白阳性表达指数和凋亡指数均明显低于模型组(P<0.05～0.01)。 结论 黄酮类成分均能对抗压力诱导的RGCs凋亡，为灯盏细辛视神经保护的有效组分。

    【关键词】 灯盏细辛黄酮类成分;视网膜神经节细胞培养;高眼压模型;细胞凋亡

    Effects of the Flavonoids of Erigeron Breviscapus on Apoptosis of Retinal Ganglion Cells by High Intraocular Pressure in vitro

    SHENG Yanmei，ZHANG Yi，MENG Xianli，LONG Yi，

    Chengdu Medical College，Chengdu，Sichuan Province610083

    ABSTRACT Objective To observe the effects of the flavonoids on apoptosis of retinal ganglion cells by high intraocular pressure in vitro and to explore the substantial foundation with neuroprotective effects in glaucoma of E.breviscapus.Methods The retina of24Sprague-Dawley rats born only for2to3days were dissociated into cell suspension with trypsin digestion.The cell suspension was implat-ed in6-well culture plates covered with hyaluronic acid and laminin in eachwell.After cultured for72hours ......