荔枝核；_抗乙肝；_黄酮类；_紫外分光光度法_

紫外分光光度法测定荔枝核抗乙肝成分的含量

http://www.100md.com 《时珍国医国药》 2006年第11期

荔枝核；,抗乙肝；,黄酮类；,紫外分光光度法,,荔枝核；,抗乙肝；,黄酮类；,紫外分光光度法,1仪器与材料,2方法与结果,3讨论,参考文献：

     摘要：目的研究荔枝核抗乙肝成分黄酮类化合物的测定方法。方法紫外分光光度法在510 nm处测定。结果建立了回归方程C(μg/ml) = 2.541 61 + 83.286 26 A, 相关系数r = 0.999 8，平均加标回收率为100.82%(RSD=1.59%)。测定荔枝核抗乙肝成分黄酮类化合物含量为7.13%。结论该方法简便，准确，可用于测定荔枝核抗乙肝成分黄酮类化合物的含量。

    关键词：荔枝核；抗乙肝；黄酮类；紫外分光光度法

    Determination of the Content of Antihepatitis B Consituents in Litchi Seed by Spectrophotometric Method

    TANG Jianping,DING Liwen, ZHOU ChunShan

    (College of Chemistry and Chemical Engineering , Jiangxi Normal University, Nanchang 330027, China; College of Chemistry and Chemical Engineering , Central South University, Changsha 410083, China)

    Abstract：ObjectiveTo establish a method for the determination of the content of antihepatitis B constituents flavonoids in Litchi Seed.MethodsThe content of anti-hapatitis B consituents flavnoids in Litchi Seed was determined by spectrophtometric method at 510nm. ResultsThe regression equation C(μg/ml) = 2.54161 + 83.28626 A and R = 0.9998 of the relationship between concentration and absorbance were established. The average recovery was 100.82% and RSD was 1.59%. The content of antihepatitis B consituents flavonoids in Litchi Seed was 7.13%. ConclusionThe method is easy and accurate, and can be used for determination of the content of antihepatitis B consituents flavnoids in Litchi Seed. ......

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