峨眉野连不同部位总生物碱和盐酸小檗碱的含量测定
峨眉野生黄连；,紫外分光光度法；,高效液相色谱；,组织培养,,峨眉野生黄连；,紫外分光光度法；,高效液相色谱；,组织培养,1仪器与材料,2方法与结果,3讨论,参考文献：
摘要：目的确定峨眉野连不同器官的总生物碱和小檗碱含量。方法采用紫外分光光度法测定总生物碱含量，高效液相色谱方法测定盐酸小檗碱含量。结果峨眉野连根茎、须根、叶片和叶柄中，均含有生物碱和小檗碱。结论 野连地上器官含有较高的总生物碱和小檗碱，对濒危植物的组织培养及保护提供理论指导。关键词：峨眉野生黄连； 紫外分光光度法； 高效液相色谱； 组织培养
Determination of Total Alkaloid and Berberine Contents in Different Organs from Coptis omeiensis
ZHOU Jiayu， LIAO Hai， PENG Shuming， CHEN Fang
(College of Pharmacy, Southwest Jiaotong University, Emei 614202, China; College of Life Science, Sichuan University, Chengdu 610064, China)
Abstract：ObjectiveTo determine the total alkaloid and berberine contents in rhizome, fibre, lamina and petiole organs from Coptis omeiensis. MethodsThe total alkaloid and berberine contents were determined by UV and HPLC methods respectively. ResultsRhizome, fibre, lamina and petiole organs obtain alkaloid and berberine contents. ConclusionThe comparatively high contents in total alkaloid and berberine in the overground organs of Coptis omeiensis may provide guidance for tissue culture and resource protection.
Key words：Coptis omeiensis; UV; HPLC; Tissue culture
黄连为重要的中药材 ......
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