复方大黄颗粒；_大黄素；_大黄酚；_高效液相色谱法_

反相高效液相色谱法测定复方大黄颗粒中大黄素和大黄酚的含量

http://www.100md.com 《时珍国医国药》 2006年第8期

复方大黄颗粒；,大黄素；,大黄酚；,高效液相色谱法,,复方大黄颗粒；,大黄素；,大黄酚；,高效液相色谱法,1仪器与试剂,2方法与结果,3讨论,参考文献：

     摘要：目的建立反相高效液相色谱(RPHPLC)法测定复方大黄颗粒中大黄素和大黄酚的含量。方法Alltima C18色谱柱(5 μm,150 mm×4.6 mm)；柱温：30℃；流动相：甲醇0.1%磷酸(87∶13)；流速：1 ml/min；检测波长：254 nm。结果大黄素的线性范围为0.096~0.576 μg(r=0.999 9)，平均回收率为99.29%，RSD为0.63%；大黄酚的线性范围为0.078 4~0.470 4 μg(r=0.999 8)，平均回收率为100.08%，RSD为1.52%。结论该方法灵敏度高、操作简便、重现性好、效率高。可作为样品的检测方法。

    关键词：复方大黄颗粒；大黄素；大黄酚；高效液相色谱法

    Determination of Emodin and Chrysoplhanol in Compound Rhubard Granula by RPHPLC

    LIANG Hengxing, YUE Ling, CAI Xujiao

    (Chengdu University of TCM, Chengdu 611730,China)

    Abstract:ObjectiveTo develop an RPHPLC method for determination of emodin and chrysophanol in Compound Rhubard Granula.MethodsThe samples were determined by RPHPLC with a Alltima C18column(5 μm,150 mm×4.6 mm) and UV detector at 254 nm.The mobile phase was methonal∶0.1%H3PO4(87∶13)at a flow rate of 1.0 ml/min.The column temperature was 30℃.ResultsThe calibration curve of emodin was linear over the ranges of 0.096μg~0.576 μg (r=0.999 9),average recovery was 99.29,RSD was 0.63%;The calibration curve of chrysophanol was linear over the ranges of 0.078 4~0.470 4 μg (r=0.999 8),average recovery was 100.08%,RSDwas 1.52%.ConclusionThis method is simple,reliable,efficient,and it provides a reliable method for determination of emodin and chrysophanol in Compound Rhubard Granula. ......

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