川芎嗪；_肠缺血再灌注损伤；_小肠；_肝脏_

川芎嗪对大鼠肠缺血再灌注肝损伤保护作用的实验研究

http://www.100md.com 《时珍国医国药》 2006年第8期

川芎嗪；,肠缺血再灌注损伤；,小肠；,肝脏,,川芎嗪；,肠缺血再灌注损伤；,小肠；,肝脏,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的探讨川芎嗪对大鼠肠缺血再灌注肝损伤的保护作用。方法选用健康Wistar大鼠24只，随机分成3组(每组8只)：假手术对照组、缺血再灌注组、川芎嗪治疗组。夹闭大鼠肠系膜上动脉(SMA)60 min造成缺血，再灌注2 h后取出肝组织制成匀浆，测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSHPX)、丙二醛(MDA)、Ca2+Mg2+ATP酶的含量及肝形态细胞学变化。结果川芎嗪治疗组肝组织MDA含量明显低于缺血再灌注组(P<0.01)，SOD，GSHPX，Ca2+Mg2+ATP酶的含量均明显高于缺血再灌注组(P<0.01)，肝细胞形态学异常变化明显减轻。结论川芎嗪能减少大鼠脂质过氧化，改善ATPase功能，减轻大鼠肠缺血再灌注所致肝脏损伤。

    关键词：川芎嗪；肠缺血再灌注损伤；小肠；肝脏

    Protective Effects of Tetramethylpyrazine on Hepatic Damage Induced by Intestinal Ischemiareperfusion in Rats

    GAO Hui, RUAN Mingfeng, BAI Yuting, WAN Jingzhi

    (Department of Surgery, Medical School of Xianning College, Xianning 437100,China; The First Affiliated Hospital of Xianning College，Xianning 437100,China)

    Abstract:ObjectiveTo explore the protective effects of tetramethylpyrazine on hepatic damage induced by intestinal ischemiareperfusion in rats. MethodsDivide the rats randomly into three groups: ⑴sham operated group, ⑵untreated ischemiareperfution(I/R) group, ⑶pretreatment with tetramethylpyrazine before I/R(Te). Superior mesenteric artery was clipped for 60 min to cause ischimia, and then unclipped to make reperfusion for 2 hours after reperfusion, the liver tissues were gathered and homogenized respectively, and the contents of SOD, GSHPX, MDA and ATPase,and morphological changes of hepatocytes were assayed.ResultsThe contents of MDA in liver in Te groups were less than that in I/R group (P<0.01),the contents of SOD,GSHPX and Ca2+,Mg2+,ATPase in Te groups were higher than those in I/R group(P<0.01),and the abnormal morphological changes of hepatocytes diminished remarkably. ConclusionTetramethylpyrazine can reduce lipid peroxidation, improve ATPase activity and decrease the injury of liver tissue in the rats after intestinal ischemiareperfusion. ......

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