感清颗粒剂；_黄芩苷；_高效液相色谱法_

高效液相色谱法测定感清颗粒剂中黄芩苷的含量

http://www.100md.com 《时珍国医国药》 2006年第8期

感清颗粒剂；,黄芩苷；,高效液相色谱法,,感清颗粒剂；,黄芩苷；,高效液相色谱法,1仪器与试药,2方法与结果,3讨论,参考文献：

     摘要：目的建立感清颗粒剂中黄芩苷含量的高效液相色谱法(HPLC)测定方法。方法采用日本岛津公司shimpack CLCODS柱(6.0 mm×150 mm)，流动相为甲醇水磷酸(47∶53∶0.2)，在280 nm波长处检测。结果黄芩苷在9.70 ～97.0 μg/ml范围内呈线性关系，r=1.000；平均加样回收率为101.2%，RSD=2.01% (n=5)。结论该方法结果准确、重现性好，可用于测定感清颗粒剂中黄芩苷的含量。

    关键词：感清颗粒剂；黄芩苷；高效液相色谱法

    Determination of Baicalin in Ganqing Granules by HPLC

    ZHANG Xinjun, LIANG Ruixue, JIA Yuanyin

    (Shandong Academy of Traditional Chinese Medicine, Jinan 250014，China)

    Abstract：ObjectiveTo establish a method for the determination of baicalin by HPLC in Ganqing Granules.MethodsShim-pack CLCODS column was used. The mobile phase was methanolwater H3PO4 (47∶53∶0.2). The detection wavelength was at 280 nm. ResultsThe linear range of Baicalin was 9.70～97.0 μg/ml ,r=1.000. The average recovery was 101.2%, RSD=2.01% (n=5).ConclusionThe result is accurate and the reproducibility is good. This method can be used for the determination of Baicalin in Ganqing Granules.

    Key words：Ganqing Granules; Baicalin; HPLC

    感清颗粒主要由黄芩、柴胡等组成 ......

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