幽门螺杆菌重组中性粒细胞激活蛋白蛋黄抗体的制备
http://www.100md.com
邓 颖, 杨致邦, 黄 伟, 林珊珊, 黄 进, 叶翠莲
幽门螺杆菌;重组蛋白;中性粒细胞激活蛋白;蛋黄抗体,邓颖,杨致邦,林珊珊,黄进,叶翠莲,黄伟,邓颖,通讯作者:,Preparationofeggyolkimmunoglobulinagainstrecombinantneutrophilac
 |
| 第1页 |
参见附件(925KB，5页)。
邓颖, 杨致邦, 林珊珊, 黄进, 叶翠莲, 重庆医科大学微生物学与免疫学教研究室 重庆市 400016
黄伟, 西南大学蚕学与生物技术学院生物技术系 重庆市 402460
邓颖, 重庆医科大学硕士, 主要从事临床消化内科及幽门螺杆菌的研究.
通讯作者: 杨致邦, 400016, 重庆市, 重庆医科大学微生物学与免疫学教研究室. dryangfm365@sina.com
电话: 023-68485193
收稿日期: 2006-01-09 接受日期: 2006-01-25
Preparation of egg yolk immunoglobulin against recombinant neutrophil activating protein in Helicobacter pylori
Ying Deng, Zhi-Bang Yang, Wei Huang, Shan-Shan Lin, Jin Huang, Cui-Lian Ye
Ying Deng, Zhi-Bang Yang, Shan-Shan Lin, Jin Huang, Cui-Lian Ye,Department of Microbiology and Immunology, Chongqing University of Medical Science, Chongqing 400016, China
Wei Huang,Department Biotechnology, College of Silkworm and Biotechnology Science, Southwest University, Chongqing 402460, China
Correspondence to:Zhi-Bang Yang, Department of Microbiology and Immunology, Chongqing University of Medical Science, Chongqing 400016, China. dryangfm365@sina.com
Received:2006-01-09 Accepted:2006-01-25
Abstract
AIM: To prepare a highly specific and efficient egg yolk immunoglobulin (IgY) against recombinant neutrophil activating protein (Nap) of Helicobacter pylori from the yolk of hen’s eggs.
METHODS: Recombinant bacteria of pQE30-NapA-DH5α were cultured in large numbers to collect Nap protein. The recombinant protein was purified by Ni2+-NAT chromatography, and its protein concentration was detected by Bradford method. After laying hens were immunized with recombinant Nap, IgY was isolated by water-dilution (WD) combined chloroform precipitation method. The relationship between IgY titer and immune time was assessed by enzyme-linked immunosorbent assay (ELISA). High-titer IgY was purified and concentrated by ammonium sulphate precipitation. The Nap-IgY titer and protein concentration were measured by indirect ELISA and Bradford method, respevtively, and the activity of Nap-IgY was identified by Western blot ......
您现在查看是摘要介绍页，详见PDF附件(925KB，5页)。