骨髓基质干细胞向肝细胞的定向分化及体内移植
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郭菲菲, 刘晓萍, 高胜利, 冯智慧
骨髓基质干细胞;肝细胞;分化;移植,郭菲菲,刘晓萍,冯智慧,高胜利,郭菲菲,通讯作者:,Differentiationintohepatocytesandtransplantationofbonemarrowstromalstemcells
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郭菲菲, 青岛大学医学院病理生理学教研室 山东省青岛市 266021
刘晓萍, 冯智慧, 青岛大学医学院组织学与胚胎学教研室 山东省青岛市 266021
高胜利, 青岛大学医学院医学研究生教育管理办公室 山东省青岛市 266021
郭菲菲, 2002年青岛大学医学院硕士, 助教, 主要从事干细胞的研究.
通讯作者: 刘晓萍, 266021, 山东省青岛市登州路38号, 青岛大学医学院组织学与胚胎学教研室. xpliu572001@yahoo.com.cn
电话: 0532-82991708
收稿日期: 2006-10-26 接受日期: 2006-11-28
Differentiation into hepatocytes and transplantation of bone marrow stromal stem cells
Fei-Fei Guo, Xiao-Ping Liu, Sheng-Li Gao, Zhi-Hui Feng
Fei-Fei Guo,Department of Pathophysiology, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Xiao-Ping Liu, Zhi-Hui Feng, Department of Histology and Embryology, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Sheng-Li Gao,Office of Postgraduate Administration, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Correspondence to:Xiao-Ping Liu, Department of Histology and Embryology, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China. xpliu572001@yahoo.com.cn
Received:2006-10-26 Accepted:2006-11-28
Abstract
AIM: To investigate the differentiation of bone marrow stromal stem cells (BMSCs) into hepatocytes in vitro and the reparation of liver cells after BMSCs transplantation in mice with liver injury.
METHODS: BMSCs were induced by hepatic extract fluid (HE) to differentiate into hepatocytes in vitro, which was identified by immunocytochemistry and Indocyanine green (ICG) staining. The BrdU-labeled BMSCs were transplanted into the mice through caudal vein, and then the their distributions were detected by immunohistochemistry. The concentration changes of aspartate aminotransferase (AST, nkat/L), alanine aminotransferase (ALT, nkat/L) and alkaline phosphatase (AKP, nkat/L) were observed by serum enzymology test on the 7th, 14th, 28th day after transplantation ......
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