骨髓间充质干细胞向肝细胞的诱导分化
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钟晓琳, 李昌平, 胡莲, 万居易
骨髓间充质干细胞;体外培养;诱导分化;肝细胞生长因子;制瘤素M;肝细胞钟晓琳,李昌平,胡莲,万居易.骨髓间充质干细胞向肝细胞的诱导分化. 世界华人消化杂志2007;15(6)568-573,钟晓琳,李昌平,胡莲,万居易,钟晓琳,通讯作
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钟晓琳, 李昌平, 泸州医学院附属医院消化科 四川省泸州市 646000
胡莲, 宜宾市第一人民医院消化科 四川省宜宾市 644000
万居易, 泸州医学院附属医院心胸外科 四川省泸州市 646000
钟晓琳, 2003年泸州医学院消化内科硕士, 医师, 主要从事肝病研究.四川省教育厅资助课题, No.2003-A027
通讯作者: 李昌平, 646000, 四川省泸州市太平街25号, 泸州医学院附属医院消化内科. lichangping1965@sina.com
电话: 0830-2392753
收稿日期: 2006-10-30 接受日期: 2006-11-28
Hepatocytic differentiation of bone marrow-derived mesenchymal stem cells
Xiao-Lin Zhong, Chang-Ping Li, Lian Hu, Ju-Yi Wan
Xiao-Lin Zhong, Chang-Ping Li, Department of Digestive Diseases, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
Lian Hu, Department of Digestive Diseases, the First People's Hospital of Yibin City, Yibin 644000, Sichuan Province, China
Ju-Yi Wan, Department of Cardiac and Thoracic Surgery, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
Supported by the Education Department Foundation of Sichuan Province, No.2003-A027
Correspondence to: Dr. Chang-Ping Li, Department of digestion, The Application Hospital Of Luzhou Medical College, 25 taiping jie, luzhou 646000, Sichuan province, china. lichangping1965@sina.com
Received: 2006-10-30 Accepted: 2006-11-28
AbstractAIM:To explore the capability and effect of hepatocyte growth factor (HGF) and oncostatin M (OSM) in inducing the differentiation of bone marrow mesenchymal stem cells (MSCs) into hepatocytes in vitro.
METHODS: Rat MSCs were isolated and cultured. Passage 3 MSCs were divided into group A, B, C, D and E, which were induced by dulbecco's modified eagle medium-low glucose (DMEM-LG) plus 100 mL/L fetal calf serum (FCS), hepatocyte growth medium (HGM), HGM plus 20 mg/L HGF, HGM plus 20 mg/L OSM, and HGM plus 20 mg/L HGF plus 20 mg/L OSM, respectively. The expression of alpha-fetoprotein (AFP) and cytokeratin 18 (CK18) were detected by immunocytochemistry; the glycogen deposit was examined by Periodic acid-Schiff (PAS) staining; and the urea content in culture supernatant was determined by glutamate dehydrogenases at different time points.
RESULTS: On the 7th day, the positive expression of AFP emerged in group C and E, and it was lower in the former than that in the latter (c2 = 6.322, P < 0.05). With the extension of induction time, the expression of AFP was decreased gradually. The expression of CK18 was found in group E and C on the 7th and 14th day, respectively, and the glycogen deposit was observed on the 7th day both in group E and C. With the extension of induction time, the levels of CK18 and glycogen expression were elevated gradually. At the same time point, the expression rates of CK18 (14 d: c2 = 4.811, P < 0.05; 21 d: c2 = 6.902, P < 0.01; 28 d: c2 = 5.771, P < 0 ......
钟晓琳, 李昌平, 泸州医学院附属医院消化科 四川省泸州市 646000
胡莲, 宜宾市第一人民医院消化科 四川省宜宾市 644000
万居易, 泸州医学院附属医院心胸外科 四川省泸州市 646000
钟晓琳, 2003年泸州医学院消化内科硕士, 医师, 主要从事肝病研究.四川省教育厅资助课题, No.2003-A027
通讯作者: 李昌平, 646000, 四川省泸州市太平街25号, 泸州医学院附属医院消化内科. lichangping1965@sina.com
电话: 0830-2392753
收稿日期: 2006-10-30 接受日期: 2006-11-28
Hepatocytic differentiation of bone marrow-derived mesenchymal stem cells
Xiao-Lin Zhong, Chang-Ping Li, Lian Hu, Ju-Yi Wan
Xiao-Lin Zhong, Chang-Ping Li, Department of Digestive Diseases, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
Lian Hu, Department of Digestive Diseases, the First People's Hospital of Yibin City, Yibin 644000, Sichuan Province, China
Ju-Yi Wan, Department of Cardiac and Thoracic Surgery, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
Supported by the Education Department Foundation of Sichuan Province, No.2003-A027
Correspondence to: Dr. Chang-Ping Li, Department of digestion, The Application Hospital Of Luzhou Medical College, 25 taiping jie, luzhou 646000, Sichuan province, china. lichangping1965@sina.com
Received: 2006-10-30 Accepted: 2006-11-28
AbstractAIM:To explore the capability and effect of hepatocyte growth factor (HGF) and oncostatin M (OSM) in inducing the differentiation of bone marrow mesenchymal stem cells (MSCs) into hepatocytes in vitro.
METHODS: Rat MSCs were isolated and cultured. Passage 3 MSCs were divided into group A, B, C, D and E, which were induced by dulbecco's modified eagle medium-low glucose (DMEM-LG) plus 100 mL/L fetal calf serum (FCS), hepatocyte growth medium (HGM), HGM plus 20 mg/L HGF, HGM plus 20 mg/L OSM, and HGM plus 20 mg/L HGF plus 20 mg/L OSM, respectively. The expression of alpha-fetoprotein (AFP) and cytokeratin 18 (CK18) were detected by immunocytochemistry; the glycogen deposit was examined by Periodic acid-Schiff (PAS) staining; and the urea content in culture supernatant was determined by glutamate dehydrogenases at different time points.
RESULTS: On the 7th day, the positive expression of AFP emerged in group C and E, and it was lower in the former than that in the latter (c2 = 6.322, P < 0.05). With the extension of induction time, the expression of AFP was decreased gradually. The expression of CK18 was found in group E and C on the 7th and 14th day, respectively, and the glycogen deposit was observed on the 7th day both in group E and C. With the extension of induction time, the levels of CK18 and glycogen expression were elevated gradually. At the same time point, the expression rates of CK18 (14 d: c2 = 4.811, P < 0.05; 21 d: c2 = 6.902, P < 0.01; 28 d: c2 = 5.771, P < 0 ......
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