IL-24对细胞因子诱导的杀伤细胞的作用
http://www.100md.com
袁玉涛, 王志华, 秦 莉, 张春艳, 马玉彦, 孙喜文
细胞因子诱导的杀伤细胞;IL-24;细胞毒活性;增殖活性袁玉涛,王志华,秦莉,张春艳,马玉彦,孙喜文.IL-24对细胞因子诱导的杀伤细胞的作用. 世界华人消化杂志2007;15(6)548-553,袁玉涛,王志华,秦莉,张春艳,马玉彦,孙喜文
参见附件(1kb)。
袁玉涛, 王志华, 秦莉, 张春艳, 马玉彦, 孙喜文, 哈尔滨医科大学肿瘤研究所 黑龙江省哈尔滨市 150081
袁玉涛, 哈尔滨医科大学研究生, 主要从事肿瘤的生物治疗的临床和实验研究.国家自然科学基金资助项目, No.39270765
黑龙江省卫生厅基金资助项目, No.2006-478
通讯作者: 王志华, 150081, 黑龙江省哈尔滨市, 哈尔滨医科大学肿瘤研究所. hljwzh000@163.com
电话: 0451-86298393 传真: 0451-86665003
收稿日期: 2006-11-21 接受日期: 2006-12-18
Effects of interleukin-24 on the cytotoxic activity of cytokine-induced killer cells
Yu-Tao Yuan, Zhi-Hua Wang, Li Qin, Chun-Yan Zhang, Yu-Yan Ma, Xi-Wen Sun
Yu-Tao Yuan, Zhi-Hua Wang, Li-Qin, Chun-Yan Zhang, Yu-Yan Ma, Xi-Wen Sun, Cancer Research Institute, Harbin Medical University, Harbin 150081, Heilongjiang Province, China
Supported by National Natural Science Foundation of China, No.39270765, and the Foundation from Health Ministry of Heilongjiang Province, No.2006-478
Correspondence to: Zhi-Hua Wang, Cancer Research Institute of Harbin Medical University, Harbin 150081, Heilongjiang Province, China. hljwzh000@163.com
Received: 2006-11-21 Accepted: 2006-12-18
AbstractAIM:To find a new method to enhance the cytotoxic activity of cytokine-induced killer (CIK) cells for clinical immunotherapy.
METHODS: Mononuclear cells were extracted from the peripheral blood of health adults. One group was treated with interferon-g (IFN-g) at the 1st day and interleukin-1 (IL-1), CD3 mAb, IL-2 at the 2nd day, and the other group was disposed with IL-24 besides IL-1, CD3 mAb, and IL-2. Cell counting method was used to determine the proliferation of CIK cells, and the cytotoxic activity was detected by MTT assay. Cell phenotype was examined by flow cytometry. The scanning electron microscopy and transmission electron microscopy were used to observe the killing effects of CIK cells on tumor cells as well as the changes of tumor cells.
RESULTS: The proliferation of CIK cells without IL-24 treatment was higher than that with IL-24 treatment (126.34 ± 2.14 vs 108.87 ± 1.29, P < 0.05). The cytotoxic activities of CIK cells co-cultured with IL-24 were above 90% at different effector-target ratios (10∶1, 20∶1, 40∶1), which were significantly higher than those in the other groups (10∶1: 95.58% ± 2.21% vs 27.31% ± 2.69%, 8.74% ± 2.41%, 38.65% ± 21.30%, all P < 0.05; 20∶1: 91.97% ± 4.21% vs 34.27% ± 0.85%, 11.54% ± 2.78%, 48.32% ± 11.72%, all P < 0.05; 40∶1: 91.84% ± 9.28% vs 50.67% ± 1.30%, 23.73% ± 11.07%, 52.89% ± 12.26%, all P < 0.05). Cell phenotypes were not significantly different between the CIK cells with and without IL-24 treatment (P > 0.05). Apoptotic and necrotic cells were obviously increased in after IL-24 treatment under transmission electron microscope.
CONCLUSION: IL-24 can obviously enhance the cytotoxic activity of CIK cell.
Key Words: Cytokine-induced killer cell; Interleukin-24; Cytotoxic activity; Proliferation ......
袁玉涛, 王志华, 秦莉, 张春艳, 马玉彦, 孙喜文, 哈尔滨医科大学肿瘤研究所 黑龙江省哈尔滨市 150081
袁玉涛, 哈尔滨医科大学研究生, 主要从事肿瘤的生物治疗的临床和实验研究.国家自然科学基金资助项目, No.39270765
黑龙江省卫生厅基金资助项目, No.2006-478
通讯作者: 王志华, 150081, 黑龙江省哈尔滨市, 哈尔滨医科大学肿瘤研究所. hljwzh000@163.com
电话: 0451-86298393 传真: 0451-86665003
收稿日期: 2006-11-21 接受日期: 2006-12-18
Effects of interleukin-24 on the cytotoxic activity of cytokine-induced killer cells
Yu-Tao Yuan, Zhi-Hua Wang, Li Qin, Chun-Yan Zhang, Yu-Yan Ma, Xi-Wen Sun
Yu-Tao Yuan, Zhi-Hua Wang, Li-Qin, Chun-Yan Zhang, Yu-Yan Ma, Xi-Wen Sun, Cancer Research Institute, Harbin Medical University, Harbin 150081, Heilongjiang Province, China
Supported by National Natural Science Foundation of China, No.39270765, and the Foundation from Health Ministry of Heilongjiang Province, No.2006-478
Correspondence to: Zhi-Hua Wang, Cancer Research Institute of Harbin Medical University, Harbin 150081, Heilongjiang Province, China. hljwzh000@163.com
Received: 2006-11-21 Accepted: 2006-12-18
AbstractAIM:To find a new method to enhance the cytotoxic activity of cytokine-induced killer (CIK) cells for clinical immunotherapy.
METHODS: Mononuclear cells were extracted from the peripheral blood of health adults. One group was treated with interferon-g (IFN-g) at the 1st day and interleukin-1 (IL-1), CD3 mAb, IL-2 at the 2nd day, and the other group was disposed with IL-24 besides IL-1, CD3 mAb, and IL-2. Cell counting method was used to determine the proliferation of CIK cells, and the cytotoxic activity was detected by MTT assay. Cell phenotype was examined by flow cytometry. The scanning electron microscopy and transmission electron microscopy were used to observe the killing effects of CIK cells on tumor cells as well as the changes of tumor cells.
RESULTS: The proliferation of CIK cells without IL-24 treatment was higher than that with IL-24 treatment (126.34 ± 2.14 vs 108.87 ± 1.29, P < 0.05). The cytotoxic activities of CIK cells co-cultured with IL-24 were above 90% at different effector-target ratios (10∶1, 20∶1, 40∶1), which were significantly higher than those in the other groups (10∶1: 95.58% ± 2.21% vs 27.31% ± 2.69%, 8.74% ± 2.41%, 38.65% ± 21.30%, all P < 0.05; 20∶1: 91.97% ± 4.21% vs 34.27% ± 0.85%, 11.54% ± 2.78%, 48.32% ± 11.72%, all P < 0.05; 40∶1: 91.84% ± 9.28% vs 50.67% ± 1.30%, 23.73% ± 11.07%, 52.89% ± 12.26%, all P < 0.05). Cell phenotypes were not significantly different between the CIK cells with and without IL-24 treatment (P > 0.05). Apoptotic and necrotic cells were obviously increased in after IL-24 treatment under transmission electron microscope.
CONCLUSION: IL-24 can obviously enhance the cytotoxic activity of CIK cell.
Key Words: Cytokine-induced killer cell; Interleukin-24; Cytotoxic activity; Proliferation ......
您现在查看是摘要介绍页，详见PDF附件(1kb)。