外周血单个核细胞TRAIL mRNA和血清sTRAIL水平与HBV感染肝损伤的相关性
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毛丽萍, 王惠民, 张子玉, 吴月平, 章幼奕, 鞠少卿, 王陆军, 陈育凤
荧光定量PCR;外周血单个核细胞;肿瘤坏死因子相关凋亡诱导配体;慢性乙型肝炎毛丽萍,王惠民,张子玉,吴月平,章幼奕,鞠少卿,王陆军,陈育凤.外周血单个核细胞TRAILmRNA和血清sTRAIL水平与HBV感染肝损伤的相关性. 世界华人消化杂志2007;15(6)641-645,外周血单个
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毛丽萍, 张子玉, 吴月平, 章幼奕, 王陆军, 陈育凤, 南通大学附属南通第三医院检验科 江苏省南通市 226006
王惠民, 鞠少卿, 南通大学附属医院检验科 江苏省南通市 226001南通市社会发展基金资助课题, No. S2006043
通讯作者: 毛丽萍, 226006, 江苏省南通市, 南通大学附属南通第三医院检验科. maoliping70@163.com
电话: 0513-85116068
收稿日期: 2006-11-09 接受日期: 2006-12-01
Involvement of tumor necrosis factor-related apoptosis-inducing ligand mRNA in peripheral blood mononuclear cells and serum soluble tumor necrosis factor-related apoptosis-inducing ligand in liver injury caused by chronic hepatitis B
Li-Ping Mao, Hui-Min Wang, Zi-Yu Zhang, Yue-Ping Wu, You-Yi Zhang, Shao-Qing Ju, Lu-Jun Wang, Yu-Feng Chen
Li-Ping Mao, Zi-Yu Zhang, Yue-Ping Wu, You-Yi Zhang, Lu-Jun Wang, Yu-Feng Chen,Department of Clinical Laboratory, the Affiliated Third Hospital of Nantong University, Nantong 226006, Jiangsu Province, China
Hui-Min Wang, Shao-Qing Ju,Department of Clinical Laboratory, the Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Supported bySocial Development Foundation of Nantong City, No. S2006043
Correspondence to:Li-Ping Mao, Department of Clinical Laboratory, the the Affiliated Third Hospital of Nantong University, Nantong 226006, Jiangsu Province,China. maoliping70@163.com
Received:2006-11-09 Accepted:2006-12-01
Abstract
AIM:To explore the correlations of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA in peripheral blood mononuclear cells (PBMCs) and serum soluble TRAIL (sTRAIL) in liver injury during chronic hepatitis B (CHB).
METHODS:Real-time fluorescence quantitative polymerase chain reaction (FQ-RT-PCR) and sandwich enzyme-linked immunoadsorbent assay were used to detect the expression of TRAIL mRNA in the PBMCs and the level of sTRAIL in serum of 58 CHB patients, respectively. Then correlation analysis was performed between the results and serum HBV DNA titer, albumin, alanine aminotransferase (ALT) and total bilirubin (TBIL) levels.
RESULTS:The serum level of sTRAIL in patients with CHB (except for the light type) was significantly lower than that in the healthy controls (t = 2.91, P < 0.05), but there were no significantly differences among patients with various types of CHB. The level of sTRAIL was positively correlated with serum albumin (r = 0.426, P < 0.05), but it had no relationship with HBV DNA titer, TBIL and ALT. The expression of TRAIL mRNA in PBMCs was obviously higher in all the CHB patients than that in the healthy controls (t = 28.31, P<0.001), and it had no marked relationship with liver function and HBV DNA titer.
CONCLUSION: Lower level of serum sTRAIL may be correlated with CHB-caused liver injury, which indicates that up-regulation of TRAIL expression on the membranes of PBMCs is involvement in the delayed course of hepatitis B.
Key Words: Tumor necrosis factor-related apoptosis-inducing ligand; Real-time fluorescence quantitative polymerase chain reaction; Peripheral blood mononuclear cell; Chronic hepatitis B ......
毛丽萍, 张子玉, 吴月平, 章幼奕, 王陆军, 陈育凤, 南通大学附属南通第三医院检验科 江苏省南通市 226006
王惠民, 鞠少卿, 南通大学附属医院检验科 江苏省南通市 226001南通市社会发展基金资助课题, No. S2006043
通讯作者: 毛丽萍, 226006, 江苏省南通市, 南通大学附属南通第三医院检验科. maoliping70@163.com
电话: 0513-85116068
收稿日期: 2006-11-09 接受日期: 2006-12-01
Involvement of tumor necrosis factor-related apoptosis-inducing ligand mRNA in peripheral blood mononuclear cells and serum soluble tumor necrosis factor-related apoptosis-inducing ligand in liver injury caused by chronic hepatitis B
Li-Ping Mao, Hui-Min Wang, Zi-Yu Zhang, Yue-Ping Wu, You-Yi Zhang, Shao-Qing Ju, Lu-Jun Wang, Yu-Feng Chen
Li-Ping Mao, Zi-Yu Zhang, Yue-Ping Wu, You-Yi Zhang, Lu-Jun Wang, Yu-Feng Chen,Department of Clinical Laboratory, the Affiliated Third Hospital of Nantong University, Nantong 226006, Jiangsu Province, China
Hui-Min Wang, Shao-Qing Ju,Department of Clinical Laboratory, the Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Supported bySocial Development Foundation of Nantong City, No. S2006043
Correspondence to:Li-Ping Mao, Department of Clinical Laboratory, the the Affiliated Third Hospital of Nantong University, Nantong 226006, Jiangsu Province,China. maoliping70@163.com
Received:2006-11-09 Accepted:2006-12-01
Abstract
AIM:To explore the correlations of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA in peripheral blood mononuclear cells (PBMCs) and serum soluble TRAIL (sTRAIL) in liver injury during chronic hepatitis B (CHB).
METHODS:Real-time fluorescence quantitative polymerase chain reaction (FQ-RT-PCR) and sandwich enzyme-linked immunoadsorbent assay were used to detect the expression of TRAIL mRNA in the PBMCs and the level of sTRAIL in serum of 58 CHB patients, respectively. Then correlation analysis was performed between the results and serum HBV DNA titer, albumin, alanine aminotransferase (ALT) and total bilirubin (TBIL) levels.
RESULTS:The serum level of sTRAIL in patients with CHB (except for the light type) was significantly lower than that in the healthy controls (t = 2.91, P < 0.05), but there were no significantly differences among patients with various types of CHB. The level of sTRAIL was positively correlated with serum albumin (r = 0.426, P < 0.05), but it had no relationship with HBV DNA titer, TBIL and ALT. The expression of TRAIL mRNA in PBMCs was obviously higher in all the CHB patients than that in the healthy controls (t = 28.31, P<0.001), and it had no marked relationship with liver function and HBV DNA titer.
CONCLUSION: Lower level of serum sTRAIL may be correlated with CHB-caused liver injury, which indicates that up-regulation of TRAIL expression on the membranes of PBMCs is involvement in the delayed course of hepatitis B.
Key Words: Tumor necrosis factor-related apoptosis-inducing ligand; Real-time fluorescence quantitative polymerase chain reaction; Peripheral blood mononuclear cell; Chronic hepatitis B ......
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