缺血-再灌流时卡巴胆碱对大鼠肠上皮细胞的保护作用
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邹晓防, 林 凯, 吕 艺, 黎君友, 陆江阳, 胡 森, 盛志勇
缺血,再灌流;卡巴胆碱;肠上皮细胞;大鼠邹晓防,林凯,吕艺,黎君友,陆江阳,胡森,盛志勇.缺血-再灌流时卡巴胆碱对大鼠肠上皮细胞的保护作用. 世界华人消化杂志2007;15(6)625-628,邹晓防,林凯,吕艺,黎君友,陆江阳,胡森,盛
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邹晓防, 林凯, 吕艺, 黎君友, 陆江阳, 胡森, 盛志勇, 中国人民解放军总医院第一附属医院烧伤研究所 北京市 100037
林凯, 空军总医院临床检验中心 北京市 100037军队十一五医药卫生专项课题项目, No. 06z055
通讯作者: 胡森, 100037, 北京市, 中国人民解放军总医院第一附属医院烧伤研究所. hs82080@yahoo.com.cn
电话: 010-66867397 传真: 010-68989139
收稿日期: 2006-11-17 接受日期: 2006-12-27
Protective effects of carbachol on intestinal epithelial cells in rats with gut ischemia-reperfusion
Xiao-Fang Zou, Kai Lin, Yi Lv, Jun-You Li, Jiang-Yang Lu, Sen Hu, Zhi-Yong Sheng
Xiao-Fang Zou, Kai Lin, Yi Lv, Jun-You Li, Jiang-Yang Lu, Sen Hu, Zhi-Yong Sheng,Burn Research Institute, the First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100037, China
Kai Lin,Center of Clinical Laboratory, General Hospital of Chinese PLA Air Force, Beijing 100037, China
Supported bythe Special Foundation of the 11th Five-Year Plan for Military Medical Project, No. 06z055
Correspondence to:Sen Hu, Burn Research Institute, the First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100037, China. hs82080@yahoo.com.cn
Received:2006-11-17 Accepted:2006-12-27
Abstract
AIM:To investigate the protective effects of carbachol on intestinal epithelial cells in rats with gut ischemia and reperfusion (I/R).
METHODS: Blood flow of superior mesenteric artery was blocked to establish I/R model in Wistar rats, and then the rats were treated with carbachol for 30, 60, 120 and 240 minutes, respectively. The content (nkat/g pro) of diamine oxidase (DAO) in gut tissue was determined by biochemical method, and the expression of tumor necrosis factor-a (TNF-a) in intestinal epithelial cells was examined by immunohistochemistry. The injuries of gut epithelial cells were assessed using Chiu’s scores.
RESULTS: The pathological changes were obviously relieved in rats treated with carbachol. The content of DAO was markedly higher in carbachol-treated rats than that in the saline controls (0 min: 42.01 ± 7.17 vs 18.50 ± 7.83; 30 min: 44.51 ± 8.00 vs 20.00 ± 5.83; 60 min: 35.67 ± 7.00 vs 16.34 ± 8.17; 120 min: 39.00 ± 7.33vs 21.84 ± 6.67; 240 min: 53.34 ± 8.17 vs 45.68 ± 6.00; all P < 0.01), while the number of TNF-a-positive cells was significantly lower (0 min: 204.4 ± 12 vs 246.4 ± 15.6; 30 min: 198.4 ± 11.2 vs 230.4 ± 14.4; 60 min: 234.4 ± 12.1 vs 270.4 ± 17.1; 120 min: 225.4 ± 10.2 vs 260.4 ± 18.5; 240 min: 196.4 ± 10.8 vs 220.4 ± 18.2; all P < 0.01).
CONCLUSION: Enteral administration of carbachol can protect intestinal epithelial cells from ischemia-reperfusion injury by decreasing the production of TNF-a.
Key Words: Ischemia/reperfusion; Carbachol; Intestinal epithelial cell; Rat
Zou XF, Lin K, Lv Y, Li JY, Lu JY, Hu S, Sheng ZY. Protective effects of carbachol on intestinal epithelial cells in rats with gut ischemia-reperfusion. Shijie Huaren Xiaohua Zazhi 2007;15(6):625-628 ......
邹晓防, 林凯, 吕艺, 黎君友, 陆江阳, 胡森, 盛志勇, 中国人民解放军总医院第一附属医院烧伤研究所 北京市 100037
林凯, 空军总医院临床检验中心 北京市 100037军队十一五医药卫生专项课题项目, No. 06z055
通讯作者: 胡森, 100037, 北京市, 中国人民解放军总医院第一附属医院烧伤研究所. hs82080@yahoo.com.cn
电话: 010-66867397 传真: 010-68989139
收稿日期: 2006-11-17 接受日期: 2006-12-27
Protective effects of carbachol on intestinal epithelial cells in rats with gut ischemia-reperfusion
Xiao-Fang Zou, Kai Lin, Yi Lv, Jun-You Li, Jiang-Yang Lu, Sen Hu, Zhi-Yong Sheng
Xiao-Fang Zou, Kai Lin, Yi Lv, Jun-You Li, Jiang-Yang Lu, Sen Hu, Zhi-Yong Sheng,Burn Research Institute, the First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100037, China
Kai Lin,Center of Clinical Laboratory, General Hospital of Chinese PLA Air Force, Beijing 100037, China
Supported bythe Special Foundation of the 11th Five-Year Plan for Military Medical Project, No. 06z055
Correspondence to:Sen Hu, Burn Research Institute, the First Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100037, China. hs82080@yahoo.com.cn
Received:2006-11-17 Accepted:2006-12-27
Abstract
AIM:To investigate the protective effects of carbachol on intestinal epithelial cells in rats with gut ischemia and reperfusion (I/R).
METHODS: Blood flow of superior mesenteric artery was blocked to establish I/R model in Wistar rats, and then the rats were treated with carbachol for 30, 60, 120 and 240 minutes, respectively. The content (nkat/g pro) of diamine oxidase (DAO) in gut tissue was determined by biochemical method, and the expression of tumor necrosis factor-a (TNF-a) in intestinal epithelial cells was examined by immunohistochemistry. The injuries of gut epithelial cells were assessed using Chiu’s scores.
RESULTS: The pathological changes were obviously relieved in rats treated with carbachol. The content of DAO was markedly higher in carbachol-treated rats than that in the saline controls (0 min: 42.01 ± 7.17 vs 18.50 ± 7.83; 30 min: 44.51 ± 8.00 vs 20.00 ± 5.83; 60 min: 35.67 ± 7.00 vs 16.34 ± 8.17; 120 min: 39.00 ± 7.33vs 21.84 ± 6.67; 240 min: 53.34 ± 8.17 vs 45.68 ± 6.00; all P < 0.01), while the number of TNF-a-positive cells was significantly lower (0 min: 204.4 ± 12 vs 246.4 ± 15.6; 30 min: 198.4 ± 11.2 vs 230.4 ± 14.4; 60 min: 234.4 ± 12.1 vs 270.4 ± 17.1; 120 min: 225.4 ± 10.2 vs 260.4 ± 18.5; 240 min: 196.4 ± 10.8 vs 220.4 ± 18.2; all P < 0.01).
CONCLUSION: Enteral administration of carbachol can protect intestinal epithelial cells from ischemia-reperfusion injury by decreasing the production of TNF-a.
Key Words: Ischemia/reperfusion; Carbachol; Intestinal epithelial cell; Rat
Zou XF, Lin K, Lv Y, Li JY, Lu JY, Hu S, Sheng ZY. Protective effects of carbachol on intestinal epithelial cells in rats with gut ischemia-reperfusion. Shijie Huaren Xiaohua Zazhi 2007;15(6):625-628 ......
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