非小细胞肺癌；Bcl-xL；基因转录；蛋白表达；细胞凋亡

Bcl-xL在非小细胞肺癌中的表达研究

http://www.100md.com 《实用医技杂志》 2006年第13期

非小细胞肺癌；Bcl-xL；基因转录；蛋白表达；细胞凋亡,,],非小细胞肺癌；Bcl-xL；基因转录；蛋白表达；细胞凋亡,1材料和方法,2结果,3讨论,参考文献：

     [摘要] 目的：观察非小细胞肺癌组织与癌旁组织中Bcl-xL的基因转录和蛋白表达水平，探讨Bcl-xL在非小细胞肺癌发生发展中的可能作用，为非小细胞肺癌的诊断和治疗提供参考。方法：反转录PCR和免疫印迹的方法分别检测60例临床确诊的非小细胞肺癌组织与癌旁组织中Bcl-xL基因表达和Bcl-xL蛋白的水平，并与肺良性病变组织比较。结果：非小细胞肺癌组织的Bcl-xL mRNA水平明显高于癌旁组织和肺良性病变组织(P<0.01)，mRNA水平与临床分期呈正相关，与其他临床病理相关因素(性别、年龄、肿瘤大小、发生部位、组织学类型、分化程度)无明显相关，而癌旁组织和肺良性病变组织Bcl-xL mRNA水平之间没有差别(P> 0.05)。蛋白表达检测结果与mRNA水平高度一致。结论：非小细胞肺癌组织中Bcl-xL基因和蛋白表达水平明显升高，可能促进了肿瘤的发生发展，可以考虑作为非小细胞肺癌临床分期和评价临床预后的参考指标。

    [关键词] 非小细胞肺癌；Bcl-xL；基因转录；蛋白表达；细胞凋亡

    Study on Bcl-xL Gene and Protein Expression in Nonsmallcell Lung Cancer

    MA Lu, CHEN XiuZhen，DONG Min

    (Shenzhen Second People Hospital, Shenzhen, Guangdong 518029, China)

    Abstract: Objective To observe the expression of Bcl-xL gene and Bcl-xL protein in nonsmallcell lung cancer tissues and adjacent tissues, and to study Bcl-xL role in the development and progression of nonsmallcell lung cancer. Methods The mRNA levels of Bcl-xL were detected by RT-PCR with its corresponding primers, and its protein expressi on levels were detected by Western Blot with mouse anti- Bcl-xL monoclonal antibody in 60 clinical confirmed nonsmallcell lung cancer tissues and their corresponding adjacent tissues (more than 5 cm far away from the tumor tissue) as well as benign lung lesion tissues from noncancer patients. Results The mRNA and protein levels of Bcl-xL were much higher in cancer tissues than adjacent tissues and benign lesion tissues (P<0.01), and expression level was positively correlated with clinical stages of the cancer (P<0.05), and was not correlated with other factors such as sex, age, tumor size, tumor location and morphological types. Well, no difference detected between adjacent tissues and benign lesion tissues (P>0.05). Conclusion Bcl-xL expression is obviously highly positive correlated with stages, which suggested that Bcl-xL may enhance the development and progression of nonsmallcell lung cancer and may could be a prognostic indicator for this kind of tumor. ......

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